Homeobox protein MSX-1; Homeobox protein Hox-7; Msh homeobox 1-like protein; MSX1; HOX7

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit Ig

Rabbit

This MSX1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 111-138 amino acids from the Central region of human MSX1.

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Vial Concentration: 0.5 (lot specific)

Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Small volumes of anti-MSX1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

This gene encodes a member of the muscle segment homeobox
gene family. The encoded protein functions as a transcriptional
repressor during embryogenesis through interactions with components
of the core transcription complex and other homeoproteins. It may
also have roles in limb-pattern formation, craniofacial
development, particularly odontogenesis, and tumor growth
inhibition. Mutations in this gene, which was once known as
homeobox 7, have been associated with nonsyndromic cleft lip with
or without cleft palate 5, Witkop syndrome, Wolf-Hirschom syndrome,
and autosomoal dominant hypodontia.

Developmental Biology; Neuroscience; Signal Transduction

Western Blot (WB), ELISA (EIA), Immunofluorescence (IF)

WB~~1:1000

Anti-MSX1 Antibody (Center)at 1:2000 dilution + mouse spleen lysates

Lysates/proteins at 20 ug per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution

Predicted band size : 31 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

Fluorescent image of U251 cell stained with MSX1 Antibody (Center). U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with MSX1 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree).MSX1 immunoreactivity is localized to Nucleus significantly.

MSX1 Antibody (Center) western blot analysis in A375 cell line lysates (35ug/lane).This demonstrates the MSX1 antibody detected the MSX1 protein (arrow).

MSX1 Antibody (Center) western blot analysis in mouse spleen tissue lysates (35ug/lane).This demonstrates the MSX1 antibody detected the MSX1 protein (arrow).

Fluorescent confocal image of SY5Y cells stained with MSX1 (Center) antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with MBS9208729 MSX1 (Center) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Note the highly specific localization of the MSX1 mainly to the nucleus.

31496

msh homeobox 1

homeobox protein MSX-1

Homeobox protein MSX-1

HOX7??[Similar Products]

MSX1_HUMAN

This gene encodes a member of the muscle segment homeobox gene family. The encoded protein functions as a transcriptional repressor during embryogenesis through interactions with components of the core transcription complex and other homeoproteins. It may also have roles in limb-pattern formation, craniofacial development, particularly odontogenesis, and tumor growth inhibition. Mutations in this gene, which was once known as homeobox 7, have been associated with nonsyndromic cleft lip with or without cleft palate 5, Witkop syndrome, Wolf-Hirschom syndrome, and autosomoal dominant hypodontia. [provided by RefSeq, Jul 2008]

MSX1: Acts as a transcriptional repressor. May play a role in limb-pattern formation. Acts in cranofacial development and specifically in odontogenesis. Expression in the developing nail bed mesenchyme is important for nail plate thickness and integrity. Expressed in the developing nail bed mesenchyme. Belongs to the Msh homeobox family.

Protein type: Motility/polarity/chemotaxis; DNA-binding

Chromosomal Location of Human Ortholog: 4p16.2

Cellular Component: nucleoplasm; transcription factor complex; cytoplasm; nucleus

Molecular Function: p53 binding

Biological Process: embryonic forelimb morphogenesis; negative regulation of striated muscle cell differentiation; transcription from RNA polymerase II promoter; muscle development; protein stabilization; in utero embryonic development; regulation of odontogenesis; mesenchymal cell proliferation; cell morphogenesis; negative regulation of transcription from RNA polymerase II promoter; middle ear morphogenesis; palate development; embryonic hindlimb morphogenesis; odontogenesis of dentine-containing teeth; anterior/posterior pattern formation; negative regulation of cell proliferation; pituitary gland development; midbrain development; positive regulation of transcription from RNA polymerase II promoter; positive regulation of DNA damage response, signal transduction by p53 class mediator; negative regulation of cell growth; positive regulation of BMP signaling pathway; negative regulation of apoptosis

Disease: Orofacial Cleft 5; Witkop Syndrome; Tooth Agenesis, Selective, 1

References for protein:
1.Sliwinski, T., et al. Cancer Epidemiol 34(5):652-655(2010)
2.Nikopensius, T., et al. Birth Defects Res. Part A Clin. Mol. Teratol. 88(9):748-756(2010)
3.Suazo, J., et al. Am. J. Med. Genet. A 152A (8), 2011-2016 (2010) :
4.Doi, T., et al. J. Pediatr. Surg. 45(6):1187-1191(2010)
5.Jugessur, A., et al. PLoS ONE 5 (7), E11493 (2010)
References for SY5Y (SH-SY5Y; ATCC#CRL-2266): 1. Ross RA, et al. Coordinate morphological and biochemical interconversion of human neuroblastoma cells. J. Natl. Cancer Inst. 71: 741-749, 1983. [PubMed: 6137586]; 2. Biedler JL, et al. Multiple neurotransmitter synthesis by human neuroblastoma cell lines and clones. Cancer Res. 38: 3751-3757, 1978. [PubMed: 29704].

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