Human Pituitary homeobox 1 (PITX1) ELISA kit; BFT; CCF; POTX; PTX1; hindlimb expressed homeobox protein backfoot; paired-like homeodomain transcription factor 1; pituitary homeo box 1; pituitary otx-related factor; paired-like homeodomain 1

For Research Use Only. Not for use in diagnostic procedures.

This assay has high sensitivity and excellent specificity for detection of human PITX1. No significant cross-reactivity or interference between human PITX1 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of PITX1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS930970 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the paired-like homeodomain 1 (PITX1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PITX1. The ELISA analytical biochemical technique of the MBS930970 kit is based on PITX1 antibody-PITX1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PITX1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PITX1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PITX1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PITX1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PITX1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PITX1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

34,128 Da

paired-like homeodomain 1

pituitary homeobox 1; pituitary homeo box 1; homeobox protein PITX1; pituitary otx-related factor; hindlimb expressed homeobox protein backfoot; hindlimb-expressed homeobox protein backfoot; paired-like homeodomain transcription factor 1

Pituitary homeobox 1

BFT; PTX1??[Similar Products]

PITX1_HUMAN

This gene encodes a member of the RIEG/PITX homeobox family, which is in the bicoid class of homeodomain proteins. Members of this family are involved in organ development and left-right asymmetry. This protein acts as a transcriptional regulator involved in basal and hormone-regulated activity of prolactin. [provided by RefSeq, Jul 2008]

PITX1: May play a role in the development of anterior structures, and in particular, the brain and facies and in specifying the identity or structure of hindlimb. Defects in PITX1 are a cause of congenital clubfoot (CCF); also known as talipes equinovarus (TEV). Clubfoot is a congenital limb deformity defined as fixation of the foot in cavus, adductus, varus, and equinus (i.e. inclined inwards, axially rotated outwards, and pointing downwards) with concomitant soft tissue abnormalities. Clubfoot may occur in isolation or as part of a syndrome. Clubfoot appears to be a multifactorial trait. Belongs to the paired homeobox family. Bicoid subfamily.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 5q31.1

Cellular Component: transcription factor complex; cytoplasm; nucleolus; nucleus

Biological Process: myoblast cell fate commitment; anatomical structure morphogenesis; transcription, DNA-dependent; pituitary gland development; cartilage development; branchiomeric skeletal muscle development; positive regulation of transcription from RNA polymerase II promoter; skeletal development; embryonic hindlimb morphogenesis

Disease: Clubfoot, Congenital, With Or Without Deficiency Of Long Bones And/or Mirror-image Polydactyly; Liebenberg Syndrome

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