Product Name
RAB27A, siRNA
Full Product Name
RAB27A siRNA (Mouse)
Product Synonym Names
Ras-related protein Rab-27A
Product Gene Name
RAB27A sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q9ERI2
Specificity
RAB27A siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse RAB27A gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of RAB27A sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
RAB27A sirna
siRNA to inhibit RAB27A expression using RNA interference
Applications Tested/Suitable for RAB27A sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for RAB27A. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001288159.1
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NCBI GenBank Nucleotide #
NM_001301230.1
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UniProt Primary Accession #
Q9ERI2
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UniProt Related Accession #
Q9ERI2[Other Products]
Molecular Weight
25,017 Da
NCBI Official Full Name
ras-related protein Rab-27A
NCBI Official Synonym Full Names
RAB27A, member RAS oncogene family
NCBI Official Symbol
Rab27a??[Similar Products]
NCBI Official Synonym Symbols
ash; 2210402C08Rik; 2410003M20Rik; 4933437C11Rik
??[Similar Products]
NCBI Protein Information
ras-related protein Rab-27A
UniProt Protein Name
Ras-related protein Rab-27A
Protein Family
Ras-related protein
UniProt Gene Name
Rab27a??[Similar Products]
UniProt Entry Name
RB27A_MOUSE
NCBI Summary for RAB27A
The protein encoded by this gene is a member of the Rab family of proteins, which is the largest family within the Ras superfamily of GTPases. This gene product is thought to regulate vesicular transport, together with its specific effectors. Mutations in this gene cause several defects, including actin-based melanosome transport defects and immunodeficiency. Mutations in the human ortholog of this gene are associated with Griscelli syndrome type 2. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]
UniProt Comments for RAB27A
RAB27A: Plays a role in cytotoxic granule exocytosis in lymphocytes. Required for both granule maturation and granule docking and priming at the immunologic synapse. Defects in RAB27A are a cause of Griscelli syndrome type 2 (GS2). Griscelli syndrome is a rare autosomal recessive disorder that results in pigmentary dilution of the skin and hair, the presence of large clumps of pigment in hair shafts, and an accumulation of melanosomes in melanocytes. GS2 patients also develop an uncontrolled T-lymphocyte and macrophage activation syndrome, known as hemophagocytic syndrome, leading to death in the absence of bone marrow transplantation. Neurological impairment is present in some patients, likely as a result of hemophagocytic syndrome. Belongs to the small GTPase superfamily. Rab family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: G protein, monomeric; G protein, monomeric, Rab
Cellular Component: Golgi apparatus; photoreceptor outer segment; membrane; secretory granule membrane; lysosome; cell; late endosome; apical plasma membrane; dendrite; melanosome; intracellular; endosome; secretory granule
Molecular Function: GTPase activity; protein domain specific binding; protein binding; GDP binding; GTP binding; myosin V binding; nucleotide binding
Biological Process: exocytosis; natural killer cell degranulation; cytotoxic T cell degranulation; melanosome localization; protein secretion; melanosome transport; vesicle-mediated transport; antigen processing and presentation; protein transport; pigment granule transport; pigmentation; small GTPase mediated signal transduction; positive regulation of phagocytosis; melanocyte differentiation; blood coagulation; Rab protein signal transduction; vesicle docking during exocytosis; pigment granule localization; positive regulation of exocytosis; protein targeting
Research Articles on RAB27A
1. Rab27b is expressed in bone marrow derived neutrophils and Rab27b knockout (Rab27b KO) along with Rab27a/b double knockout (Rab27DKO) neutrophils exhibit impaired transwell migration in vitro in response to chemokines MIP-2 and LTB4.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
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