Full Product Name
COG5 siRNA (Mouse)
Product Synonym Names
Conserved oligomeric Golgi complex subunit 5; COG complex subunit 5; Component of oligomeric Golgi complex 5
Product Gene Name
COG5 sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q8C0L8
Specificity
COG5 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse COG5 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of COG5 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
COG5 sirna
siRNA to inhibit COG5 expression using RNA interference
Applications Tested/Suitable for COG5 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for COG5. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001156598.1
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NCBI GenBank Nucleotide #
NM_001163126.1
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UniProt Primary Accession #
Q8C0L8
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UniProt Secondary Accession #
E9QM30[Other Products]
UniProt Related Accession #
Q8C0L8[Other Products]
Molecular Weight
91,391 Da
NCBI Official Full Name
conserved oligomeric Golgi complex subunit 5
NCBI Official Synonym Full Names
component of oligomeric golgi complex 5
NCBI Official Symbol
Cog5??[Similar Products]
NCBI Official Synonym Symbols
GTC90; C87247; D18362; GOLTC1; 5430405C01Rik
??[Similar Products]
NCBI Protein Information
conserved oligomeric Golgi complex subunit 5
UniProt Protein Name
Conserved oligomeric Golgi complex subunit 5
UniProt Synonym Protein Names
Component of oligomeric Golgi complex 5
Protein Family
Conserved oligomeric Golgi complex
UniProt Gene Name
Cog5??[Similar Products]
UniProt Synonym Gene Names
COG complex subunit 5??[Similar Products]
UniProt Entry Name
COG5_MOUSE
UniProt Comments for COG5
COG5: Required for normal Golgi function. Defects in COG5 are the cause of congenital disorder of glycosylation type 2I (CDG2I). A multisystem disorder caused by a defect in glycoprotein biosynthesis and characterized by under-glycosylated serum glycoproteins. Congenital disorders of glycosylation result in a wide variety of clinical features, such as defects in the nervous system development, psychomotor retardation, dysmorphic features, hypotonia, coagulation disorders, and immunodeficiency. The broad spectrum of features reflects the critical role of N-glycoproteins during embryonic development, differentiation, and maintenance of cell functions. Congenital disorder of glycosylation type 2I is characterized by mild neurological impairments. Belongs to the COG5 family. 3 isoforms of the human protein are produced by alternative splicing.
Cellular Component: nucleoplasm; Golgi apparatus; membrane; cytoplasm; Golgi transport complex
Biological Process: intra-Golgi vesicle-mediated transport; protein transport; transport; inter-Golgi cisterna vesicle-mediated transport
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
