Product Name
ASH2L, siRNA
Full Product Name
ASH2L siRNA (Mouse)
Product Synonym Names
Set1/Ash2 histone methyltransferase complex subunit ASH2; ASH2-like protein
Product Gene Name
ASH2L sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q91X20
Specificity
ASH2L siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse ASH2L gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of ASH2L sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
ASH2L sirna
siRNA to inhibit ASH2L expression using RNA interference
Applications Tested/Suitable for ASH2L sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for ASH2L. It may not necessarily be applicable to this product.
NCBI Accession #
NP_035921.2
[Other Products]
NCBI GenBank Nucleotide #
NM_011791.3
[Other Products]
UniProt Primary Accession #
Q91X20
[Other Products]
UniProt Secondary Accession #
Q3UIF9; Q9Z2X4[Other Products]
UniProt Related Accession #
Q91X20[Other Products]
Molecular Weight
68,250 Da
NCBI Official Full Name
set1/Ash2 histone methyltransferase complex subunit ASH2 isoform a
NCBI Official Synonym Full Names
ash2 (absent, small, or homeotic)-like (Drosophila)
NCBI Official Symbol
Ash2l??[Similar Products]
NCBI Protein Information
set1/Ash2 histone methyltransferase complex subunit ASH2
UniProt Protein Name
Set1/Ash2 histone methyltransferase complex subunit ASH2
UniProt Synonym Protein Names
ASH2-like protein
UniProt Gene Name
Ash2l??[Similar Products]
UniProt Entry Name
ASH2L_MOUSE
UniProt Comments for ASH2L
ASH2L: Component of the Set1/Ash2 histone methyltransferase (HMT) complex, a complex that specifically methylates 'Lys-4' of histone H3, but not if the neighboring 'Lys-9' residue is already methylated. As part of the MLL1/MLL complex it is involved in methylation and dimethylation at 'Lys-4' of histone H3. May function as a transcriptional regulator. May play a role in hematopoiesis. Interacts with HCFC1. Core component of several methyltransferase-containing complexes including MLL1/MLL, ASCOM, MLL2/MLL3 and MLL3/MLL4. Each complex is at least composed of ASH2L, RBBP5, DPY30, WDR5, one or more specific histone methyltransferases (MLL, MLL2, MLL3 and MLL4), and the facultative components C16orf53/PA1, C17orf49, CHD8, E2F6, HCFC1, HCFC2, HSP70, INO80C, KDM6A, KANSL1, LAS1L, MAX, MCRS1, MEN1, MGA, KAT8/MOF, NCOA6, PAXIP1/PTIP, PELP1, PHF20, PRP31, RING2, RUVB1/TIP49A, RUVB2/TIP49B, SENP3, TAF1, TAF4, TAF6, TAF7, TAF9 and TEX10. Interacts with DPY30 and RBBP5; the interaction is direct. Component of the SET1 complex, at least composed of the catalytic subunit (SETD1A or SETD1B), WDR5, WDR82, RBBP5, ASH2L/ASH2, CXXC1/CFP1, HCFC1 and DPY30. Interacts with SETD1A and SETD1B. Ubiquitously expressed. Predominantly expressed in ***** heart and testis and fetal lung and liver, with barely detectable expression in ***** lung, liver, kidney, prostate, and peripheral leukocytes. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Transcription regulation; Methyltransferase
Cellular Component: histone methyltransferase complex; nucleus
Molecular Function: protein binding; DNA binding; histone lysine N-methyltransferase activity (H3-K4 specific); metal ion binding
Biological Process: regulation of transcription, DNA-dependent; transcription, DNA-dependent; response to estrogen stimulus; chromatin-mediated maintenance of transcription; positive regulation of cell proliferation; histone H3-K4 methylation; positive regulation of transcription from RNA polymerase II promoter; chromatin modification; response to DNA damage stimulus
Research Articles on ASH2L
1. ASH2L enhances ERalpha expression as a coactivator of GATA3 in breast cancers
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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