Purified Mouse Monoclonal Antibody (Mab); Ras-related protein Rab-27A; Rab-27; GTP-binding protein Ram; RAB27A; RAB27

For Research Use Only. Not for use in diagnostic procedures.

Monoclonal

Mouse

Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Small volumes of anti-RAB27A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Plays a role in cytotoxic granule exocytosis in lymphocytes. Required for both granule maturation and granule docking and priming at the immunologic synapse.

Crown Antibodies; Signal Transduction

Western Blot (WB)

WB~~1:2000

Anti-RAB27A Antibody at 1:2000 dilution + K562 whole cell lysate
Lysates/proteins at 20 ug per lane.
Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 25 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.

24,041 Da

RAB27A, member RAS oncogene family

ras-related protein Rab-27A

Ras-related protein Rab-27A

RAB27; Rab-27??[Similar Products]

RB27A_HUMAN

The protein encoded by this gene belongs to the small GTPase superfamily, Rab family. The protein is membrane-bound and may be involved in protein transport and small GTPase mediated signal transduction. Mutations in this gene are associated with Griscelli syndrome type 2. Alternative splicing occurs at this locus and four transcript variants encoding the same protein have been identified. [provided by RefSeq, Jul 2008]

RAB27A: Plays a role in cytotoxic granule exocytosis in lymphocytes. Required for both granule maturation and granule docking and priming at the immunologic synapse. Defects in RAB27A are a cause of Griscelli syndrome type 2 (GS2). Griscelli syndrome is a rare autosomal recessive disorder that results in pigmentary dilution of the skin and hair, the presence of large clumps of pigment in hair shafts, and an accumulation of melanosomes in melanocytes. GS2 patients also develop an uncontrolled T-lymphocyte and macrophage activation syndrome, known as hemophagocytic syndrome, leading to death in the absence of bone marrow transplantation. Neurological impairment is present in some patients, likely as a result of hemophagocytic syndrome. Belongs to the small GTPase superfamily. Rab family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: G protein, monomeric, Rab; G protein, monomeric

Chromosomal Location of Human Ortholog: 15q15-q21.1

Cellular Component: apical plasma membrane; dendrite; Golgi apparatus; late endosome; lysosome; melanosome; photoreceptor outer segment; secretory granule; secretory granule membrane

Molecular Function: GDP binding; GTP binding; GTPase activity; myosin V binding; protein binding; protein domain specific binding

Biological Process: antigen processing and presentation; blood coagulation; cellular protein metabolic process; cytotoxic T cell degranulation; exocytosis; melanocyte differentiation; melanosome localization; melanosome transport; natural killer cell degranulation; positive regulation of exocytosis; positive regulation of phagocytosis; protein targeting; small GTPase mediated signal transduction; synaptic vesicle transport

Disease: Griscelli Syndrome, Type 2

Seabra M.C.,et al.J. Biol. Chem. 270:24420-24427(1995). Seabra M.C.,et al.Submitted (MAY-1999) to the EMBL/GenBank/DDBJ databases. Chen D.,et al.Biochem. Mol. Med. 60:27-37(1997). Tolmachova T.,et al.Gene 239:109-116(1999). Hu R.-M.,et al.Proc. Natl. Acad. Sci. U.S.A. 97:9543-9548(2000).

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