Full Product Name
MMAB siRNA (Mouse)
Product Synonym Names
Cob(I)yrinic acid a.c-diamide adenosyltransferase mitochondrial; Cob(I)alamin adenosyltransferase; Methylmalonic aciduria type B homolog
Product Gene Name
MMAB sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q9D273
Specificity
MMAB siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse MMAB gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of MMAB sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
MMAB sirna
siRNA to inhibit MMAB expression using RNA interference
Applications Tested/Suitable for MMAB sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for MMAB. It may not necessarily be applicable to this product.
NCBI Accession #
NP_084232.1
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NCBI GenBank Nucleotide #
NM_029956.3
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UniProt Primary Accession #
Q9D273
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UniProt Secondary Accession #
Q3UFL6; Q8CBZ0; Q8VBZ4[Other Products]
UniProt Related Accession #
Q9D273[Other Products]
Molecular Weight
28,233 Da
NCBI Official Full Name
cob(I)yrinic acid a,c-diamide adenosyltransferase, mitochondrial
NCBI Official Synonym Full Names
methylmalonic aciduria (cobalamin deficiency) type B homolog (human)
NCBI Official Symbol
Mmab??[Similar Products]
NCBI Official Synonym Symbols
ATR; 9130222L19Rik
??[Similar Products]
NCBI Protein Information
cob(I)yrinic acid a,c-diamide adenosyltransferase, mitochondrial
UniProt Protein Name
Cob(I)yrinic acid a,c-diamide adenosyltransferase, mitochondrial
UniProt Synonym Protein Names
Cob(I)alamin adenosyltransferase; Methylmalonic aciduria type B homolog
UniProt Gene Name
Mmab??[Similar Products]
UniProt Entry Name
MMAB_MOUSE
UniProt Comments for MMAB
MMAB: Defects in MMAB are the cause of methylmalonic aciduria type cblB (MMAB); also known as methylmalonic aciduria type B or vitamin B12-responsive methylmalonicaciduria of cblB complementation type. MMAB is a disorder of methylmalonate and cobalamin metabolism due to defective synthesis of adenosylcobalamin. Inheritance is autosomal recessive. Belongs to the Cob(I)alamin adenosyltransferase family.
Protein type: Cofactor and Vitamin Metabolism - porphyrin and chlorophyll; Transferase; Motility/polarity/chemotaxis; EC 2.5.1.17
Cellular Component: mitochondrion
Molecular Function: transferase activity; cob(I)yrinic acid a,c-diamide adenosyltransferase activity; nucleotide binding; ATP binding
Biological Process: cobalamin metabolic process
Research Articles on MMAB
1. The mouse methylmalonic aciduria- related genes, Mmaa, Mmab, and Mut may have specialized functions depending on the tissue or cell type.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
