Full Product Name
MMAB antibody
Product Synonym Names
Monoclonal MMAB; Anti-MMAB; Methylmalonic aciduria cblB type; cblB; Cob(I)alamin adenosyltransferase; Methylmalonic aciduria type B protein; ATR; MGC20496
Product Gene Name
anti-MMAB antibody
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Purity/Purification
MMAB antibody was purified by affinity chromatography.
Form/Format
Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Concentration
500 ug-1 mg/ml (lot specific)
Biological Significance
MMAB catalyzes the final step in the conversion of vitamin B(12) into adenosylcobalamin(AdoCbl), a vitamin B12-containing coenzyme for methylmalonyl-CoA mutase. Mutations in the gene are the cause of vitamin B12-dependent methylmalonic aciduria linked to the cblB complementation group.
Immunogen
MMAB antibody was raised in mouse using a full length recombinant protein of human MMAB (NP_443077) produced in HEK293T cells, as the immunogen.
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Other Notes
Small volumes of anti-MMAB antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-MMAB antibody
Mouse monoclonal MMAB antibody
Product Categories/Family for anti-MMAB antibody
Proteases, Inhibitors, & Enzymes
Applications Tested/Suitable for anti-MMAB antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB)
Application Notes for anti-MMAB antibody
FC: 1:100
IF: 1:100
WB: 1:2000
Flow Cytometry (FC/FACS) of anti-MMAB antibody
Flow Cytometric analysis of HEK293T cells transfected with either recombinant MMAB protein (red) or empty vector (blue) stained using MMAB antibody
Western Blot (WB) of anti-MMAB antibody
Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant MMAB protein (Right) or empty vector (Left) detected with MMAB antibody
Immunofluorescence (IF) of anti-MMAB antibody
Immunofluorescent staining of COS7 cells transiently transfected with recombinant MMAB protein using MMAB antibody
NCBI/Uniprot data below describe general gene information for MMAB. It may not necessarily be applicable to this product.
NCBI Accession #
AKI72121.1
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UniProt Secondary Accession #
Q9BSH0; C5HU05[Other Products]
UniProt Related Accession #
Q96EY8[Other Products]
Molecular Weight
27,388 Da
NCBI Official Full Name
MMAB, partial
NCBI Official Synonym Full Names
methylmalonic aciduria (cobalamin deficiency) cblB type
NCBI Official Symbol
MMAB??[Similar Products]
NCBI Official Synonym Symbols
ATR; cob; cblB; CFAP23
??[Similar Products]
NCBI Protein Information
cob(I)yrinic acid a,c-diamide adenosyltransferase, mitochondrial
UniProt Protein Name
Cob(I)yrinic acid a,c-diamide adenosyltransferase, mitochondrial
UniProt Synonym Protein Names
Cob(I)alamin adenosyltransferase; Methylmalonic aciduria type B protein
UniProt Gene Name
MMAB??[Similar Products]
UniProt Entry Name
MMAB_HUMAN
NCBI Summary for MMAB
This gene encodes a protein that catalyzes the final step in the conversion of vitamin B(12) into adenosylcobalamin (AdoCbl), a vitamin B12-containing coenzyme for methylmalonyl-CoA mutase. Mutations in the gene are the cause of vitamin B12-dependent methylmalonic aciduria linked to the cblB complementation group. Alternatively spliced transcript variants have been found. [provided by RefSeq, Apr 2011]
UniProt Comments for MMAB
MMAB: Defects in MMAB are the cause of methylmalonic aciduria type cblB (MMAB); also known as methylmalonic aciduria type B or vitamin B12-responsive methylmalonicaciduria of cblB complementation type. MMAB is a disorder of methylmalonate and cobalamin metabolism due to defective synthesis of adenosylcobalamin. Inheritance is autosomal recessive. Belongs to the Cob(I)alamin adenosyltransferase family.
Protein type: EC 2.5.1.17; Motility/polarity/chemotaxis; Cofactor and Vitamin Metabolism - porphyrin and chlorophyll; Transferase
Chromosomal Location of Human Ortholog: 12q24
Cellular Component: mitochondrial matrix
Molecular Function: cob(I)yrinic acid a,c-diamide adenosyltransferase activity; ATP binding
Biological Process: vitamin metabolic process; cobalamin biosynthetic process; cobalamin metabolic process; water-soluble vitamin metabolic process
Disease: Methylmalonic Aciduria, Cblb Type
Research Articles on MMAB
1. A [p.C4X]), were identified in all members of the cblB cohort.">MMAB mutations, including one novel nonsense mutation (c.12 C>A [p.C4X]), were identified in all members of the cblB cohort.
Precautions
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