Full Product Name
MMAB Antibody (Center)
Product Synonym Names
Cob(I)yrinic acid a,c-diamide adenosyltransferase; mitochondrial; Cob(I)alamin adenosyltransferase; Methylmalonic aciduria type B protein; MMAB
Product Gene Name
anti-MMAB antibody
[Similar Products]
Antibody/Peptide Pairs
MMAB peptide (MBS9229193) is used for blocking the activity of MMAB antibody (MBS9214535)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q96EY8
Species Reactivity
Human (Predicted Reactivity: Mouse)
Specificity
This MMAB antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 50-81 amino acids from the Central region of human MMAB.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (lot specific)
Antigen Type
Synthetic Peptide
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Other Notes
Small volumes of anti-MMAB antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Product Categories/Family for anti-MMAB antibody
Crown Antibodies; Metabolism; Signal Transduction
Applications Tested/Suitable for anti-MMAB antibody
Western Blot (WB), ELISA (EIA)
Application Notes for anti-MMAB antibody
WB~~1:1000
Western Blot (WB) of anti-MMAB antibody
Western blot analysis of lysate from HepG2 cell line, using MMAB Antibody (Center). MBS9214535 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.
NCBI/Uniprot data below describe general gene information for MMAB. It may not necessarily be applicable to this product.
NCBI Accession #
NP_443077.1
[Other Products]
NCBI GenBank Nucleotide #
NM_052845.3
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UniProt Primary Accession #
Q96EY8
[Other Products]
UniProt Secondary Accession #
Q9BSH0; C5HU05[Other Products]
UniProt Related Accession #
Q96EY8[Other Products]
NCBI Official Full Name
cob(I)yrinic acid a,c-diamide adenosyltransferase, mitochondrial
NCBI Official Synonym Full Names
methylmalonic aciduria (cobalamin deficiency) cblB type
NCBI Official Symbol
MMAB??[Similar Products]
NCBI Official Synonym Symbols
ATR; cob; cblB; CFAP23
??[Similar Products]
NCBI Protein Information
cob(I)yrinic acid a,c-diamide adenosyltransferase, mitochondrial
UniProt Protein Name
Cob(I)yrinic acid a,c-diamide adenosyltransferase, mitochondrial
UniProt Synonym Protein Names
Cob(I)alamin adenosyltransferase; Methylmalonic aciduria type B protein
UniProt Gene Name
MMAB??[Similar Products]
UniProt Entry Name
MMAB_HUMAN
NCBI Summary for MMAB
This gene encodes a protein that catalyzes the final step in the conversion of vitamin B(12) into adenosylcobalamin (AdoCbl), a vitamin B12-containing coenzyme for methylmalonyl-CoA mutase. Mutations in the gene are the cause of vitamin B12-dependent methylmalonic aciduria linked to the cblB complementation group. Alternatively spliced transcript variants have been found. [provided by RefSeq, Apr 2011]
UniProt Comments for MMAB
MMAB: Defects in MMAB are the cause of methylmalonic aciduria type cblB (MMAB); also known as methylmalonic aciduria type B or vitamin B12-responsive methylmalonicaciduria of cblB complementation type. MMAB is a disorder of methylmalonate and cobalamin metabolism due to defective synthesis of adenosylcobalamin. Inheritance is autosomal recessive. Belongs to the Cob(I)alamin adenosyltransferase family.
Protein type: EC 2.5.1.17; Transferase; Cofactor and Vitamin Metabolism - porphyrin and chlorophyll; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 12q24
Cellular Component: mitochondrial matrix
Molecular Function: cob(I)yrinic acid a,c-diamide adenosyltransferase activity; ATP binding
Biological Process: vitamin metabolic process; cobalamin biosynthetic process; cobalamin metabolic process; water-soluble vitamin metabolic process
Disease: Methylmalonic Aciduria, Cblb Type
Product References and Citations for anti-MMAB antibody
Dobson C.M.,et al.Hum. Mol. Genet. 11:3361-3369(2002).
Leal N.A.,et al.J. Biol. Chem. 278:9227-9234(2003).
Burkard T.R.,et al.BMC Syst. Biol. 5:17-17(2011).
Schubert H.L.,et al.Biochemistry 45:15188-15196(2006).
Martinez M.A.,et al.Mol. Genet. Metab. 84:317-325(2005).
Research Articles on MMAB
1. A [p.C4X]), were identified in all members of the cblB cohort.">MMAB mutations, including one novel nonsense mutation (c.12 C>A [p.C4X]), were identified in all members of the cblB cohort.
Precautions
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