Full Product Name
MMAB antibody
Product Synonym Names
Monoclonal MMAB; Anti-MMAB; Methylmalonic aciduria cblB type; cblB; Cob(I)alamin adenosyltransferase; Methylmalonic aciduria type B protein; ATR; MGC20496
Product Gene Name
anti-MMAB antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Purity/Purification
MMAB antibody was purified by affinity chromatography.
Form/Format
Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Concentration
500 ug-1 mg/ml (lot specific)
Biological Significance
MMAB catalyzes the final step in the conversion of vitamin B(12) into adenosylcobalamin(AdoCbl), a vitamin B12-containing coenzyme for methylmalonyl-CoA mutase. Mutations in the gene are the cause of vitamin B12-dependent methylmalonic aciduria linked to the cblB complementation group.
Immunogen
MMAB antibody was raised in mouse using a full length recombinant protein of human MMAB (NP_443077) produced in HEK293T cells, as the immunogen.
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Other Notes
Small volumes of anti-MMAB antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-MMAB antibody
Mouse monoclonal MMAB antibody
Product Categories/Family for anti-MMAB antibody
Proteases, Inhibitors, & Enzymes
Applications Tested/Suitable for anti-MMAB antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB)
Application Notes for anti-MMAB antibody
FC: 1:100
IF: 1:100
WB: 1:2000
Flow Cytometry (FC/FACS) of anti-MMAB antibody
Flow Cytometric analysis of HEK293T cells transfected with either recombinant MMAB protein (red) or empty vector (blue) stained using MMAB antibody
Western Blot (WB) of anti-MMAB antibody
Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant MMAB protein (Right) or empty vector (Left) detected with MMAB antibody
Immunofluorescence (IF) of anti-MMAB antibody
Immunofluorescent staining of COS7 cells transiently transfected with recombinant MMAB protein using MMAB antibody
NCBI/Uniprot data below describe general gene information for MMAB. It may not necessarily be applicable to this product.
NCBI Accession #
AKI72121.1
[Other Products]
UniProt Secondary Accession #
Q9BSH0; C5HU05[Other Products]
UniProt Related Accession #
Q96EY8[Other Products]
Molecular Weight
27,388 Da
NCBI Official Full Name
MMAB, partial
NCBI Official Synonym Full Names
methylmalonic aciduria (cobalamin deficiency) cblB type
NCBI Official Symbol
MMAB??[Similar Products]
NCBI Official Synonym Symbols
ATR; cob; cblB; CFAP23
??[Similar Products]
NCBI Protein Information
cob(I)yrinic acid a,c-diamide adenosyltransferase, mitochondrial
UniProt Protein Name
Cob(I)yrinic acid a,c-diamide adenosyltransferase, mitochondrial
UniProt Synonym Protein Names
Cob(I)alamin adenosyltransferase; Methylmalonic aciduria type B protein
UniProt Gene Name
MMAB??[Similar Products]
UniProt Entry Name
MMAB_HUMAN
NCBI Summary for MMAB
This gene encodes a protein that catalyzes the final step in the conversion of vitamin B(12) into adenosylcobalamin (AdoCbl), a vitamin B12-containing coenzyme for methylmalonyl-CoA mutase. Mutations in the gene are the cause of vitamin B12-dependent methylmalonic aciduria linked to the cblB complementation group. Alternatively spliced transcript variants have been found. [provided by RefSeq, Apr 2011]
UniProt Comments for MMAB
MMAB: Defects in MMAB are the cause of methylmalonic aciduria type cblB (MMAB); also known as methylmalonic aciduria type B or vitamin B12-responsive methylmalonicaciduria of cblB complementation type. MMAB is a disorder of methylmalonate and cobalamin metabolism due to defective synthesis of adenosylcobalamin. Inheritance is autosomal recessive. Belongs to the Cob(I)alamin adenosyltransferase family.
Protein type: Motility/polarity/chemotaxis; EC 2.5.1.17; Cofactor and Vitamin Metabolism - porphyrin and chlorophyll; Transferase
Chromosomal Location of Human Ortholog: 12q24
Cellular Component: mitochondrial matrix
Molecular Function: cob(I)yrinic acid a,c-diamide adenosyltransferase activity; ATP binding
Biological Process: vitamin metabolic process; cobalamin metabolic process; cobalamin biosynthetic process; water-soluble vitamin metabolic process
Disease: Methylmalonic Aciduria, Cblb Type
Research Articles on MMAB
1. A [p.C4X]), were identified in all members of the cblB cohort.">MMAB mutations, including one novel nonsense mutation (c.12 C>A [p.C4X]), were identified in all members of the cblB cohort.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
