Ras-related protein Rab-27A; Rab-27; GTP-binding protein Ram; RAB27A; RAB27

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit Ig

Rabbit

This RAB27A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 136-164 amino acids from the C-terminal region of human RAB27A.

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Small volumes of anti-RAB27A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

The protein encoded by this gene belongs to the small
GTPase superfamily, Rab family. The protein is membrane-bound and
may be involved in protein transport and small GTPase mediated
signal transduction. Mutations in this gene are associated with
Griscelli syndrome type 2. Alternative splicing occurs at this
locus and four transcript variants encoding the same protein have
been identified.

Signal Transduction

Western Blot (WB), ELISA (EIA)

WB~~1:1000

Western blot analysis of lysate from K562 cell line, using RAB27A Antibody (C-term). MBS9206799 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 35ug.

24868

RAB27A, member RAS oncogene family

ras-related protein Rab-27A

Ras-related protein Rab-27A

RAB27; Rab-27??[Similar Products]

RB27A_HUMAN

The protein encoded by this gene belongs to the small GTPase superfamily, Rab family. The protein is membrane-bound and may be involved in protein transport and small GTPase mediated signal transduction. Mutations in this gene are associated with Griscelli syndrome type 2. Alternative splicing occurs at this locus and four transcript variants encoding the same protein have been identified. [provided by RefSeq, Jul 2008]

RAB27A: Plays a role in cytotoxic granule exocytosis in lymphocytes. Required for both granule maturation and granule docking and priming at the immunologic synapse. Defects in RAB27A are a cause of Griscelli syndrome type 2 (GS2). Griscelli syndrome is a rare autosomal recessive disorder that results in pigmentary dilution of the skin and hair, the presence of large clumps of pigment in hair shafts, and an accumulation of melanosomes in melanocytes. GS2 patients also develop an uncontrolled T-lymphocyte and macrophage activation syndrome, known as hemophagocytic syndrome, leading to death in the absence of bone marrow transplantation. Neurological impairment is present in some patients, likely as a result of hemophagocytic syndrome. Belongs to the small GTPase superfamily. Rab family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: G protein, monomeric; G protein, monomeric, Rab

Chromosomal Location of Human Ortholog: 15q15-q21.1

Cellular Component: Golgi apparatus; photoreceptor outer segment; lysosome; secretory granule membrane; dendrite; apical plasma membrane; late endosome; melanosome; secretory granule

Molecular Function: GTPase activity; protein domain specific binding; protein binding; GDP binding; GTP binding; myosin V binding

Biological Process: exocytosis; natural killer cell degranulation; cytotoxic T cell degranulation; melanosome localization; protein secretion; synaptic vesicle transport; melanosome transport; antigen processing and presentation; intracellular protein transport; cellular protein metabolic process; positive regulation of phagocytosis; melanocyte differentiation; blood coagulation; Rab protein signal transduction; vesicle docking during exocytosis; protein targeting; positive regulation of exocytosis

Disease: Griscelli Syndrome, Type 2

Ohbayashi, N., et al. Pigment Cell Melanoma Res 23(3):365-374(2010)
Davila, S., et al. Genes Immun. 11(3):232-238(2010)
Meeths, M., et al. Pediatr Blood Cancer 54(4):563-572(2010)
Chattopadhyay, I., et al. Mutat. Res. 696(2):130-138(2010)
Wood, S.M., et al. Blood 114(19):4117-4127(2009)

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