RAB27; Ras-related protein Rab-27A; Rab-27; GTP-binding protein Ram

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit

Recognizes endogenous levels of RAB27 protein.

The antibody was purified by immunogen affinity chromatography.

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.

Small volumes of anti-RAB27A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Rabbit polyclonal antibody to RAB27

Western Blot (WB), Immunohistochemistry (IHC)

Western Blot: 1/500 - 1/2000, Immunohistochemistry: 1/50 - 1/200

Western blot analysis of RAB27 expression in Hela (A), U937 (B), Jurkat (C) whole cell lysates.

Immunohistochemical analysis of RAB27 staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

24,041 Da

RAB27A, member RAS oncogene family

ras-related protein Rab-27A

Ras-related protein Rab-27A

RAB27; Rab-27??[Similar Products]

RB27A_HUMAN

The protein encoded by this gene belongs to the small GTPase superfamily, Rab family. The protein is membrane-bound and may be involved in protein transport and small GTPase mediated signal transduction. Mutations in this gene are associated with Griscelli syndrome type 2. Alternative splicing occurs at this locus and four transcript variants encoding the same protein have been identified. [provided by RefSeq, Jul 2008]

RAB27A: Plays a role in cytotoxic granule exocytosis in lymphocytes. Required for both granule maturation and granule docking and priming at the immunologic synapse. Defects in RAB27A are a cause of Griscelli syndrome type 2 (GS2). Griscelli syndrome is a rare autosomal recessive disorder that results in pigmentary dilution of the skin and hair, the presence of large clumps of pigment in hair shafts, and an accumulation of melanosomes in melanocytes. GS2 patients also develop an uncontrolled T-lymphocyte and macrophage activation syndrome, known as hemophagocytic syndrome, leading to death in the absence of bone marrow transplantation. Neurological impairment is present in some patients, likely as a result of hemophagocytic syndrome. Belongs to the small GTPase superfamily. Rab family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: G protein, monomeric, Rab; G protein, monomeric

Chromosomal Location of Human Ortholog: 15q15-q21.1

Cellular Component: dendrite; late endosome; lysosome; melanosome; secretory granule

Molecular Function: GDP binding; GTP binding; GTPase activity; protein binding

Biological Process: antigen processing and presentation; exocytosis; melanosome localization; melanosome transport; positive regulation of exocytosis; positive regulation of phagocytosis; synaptic vesicle transport

Disease: Griscelli Syndrome, Type 2

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