Product Name
Protein Tyrosine Phosphatase, Non-receptor Type 14, catalytic domain (PTPN14), Recombinant Protein
Full Product Name
Protein Tyrosine Phosphatase, Non-receptor Type 14, catalytic domain, Recombinant, Human (PTP-D2, Pez, PTP36, PTPN14, Cytoskeletal-associated PTP)
Product Gene Name
PTPN14 recombinant protein
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 1; NC_000001.10 (214521640..214725024, complement). Location: 1q32.2
3D Structure
ModBase 3D Structure for Q15678
Host
Recombinant, Human from E Coli
Purity/Purification
>90% by SDS-PAGE.
Form/Format
25 mM Tris-HCl, pH 8.0, 75 mM sodium chloride, 0.05% Tween-20, 50% glycerol, 2mM EDTA, 5 mM DTT.
Specific Activity
2.6 U/ug. One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30 degree C.
Assay Buffer
50 mM HEPES, pH 7.4, 2 mM EDTA, 3mM DTT, 100 mM NaCl, 50 mM pNPP.
Preparation and Storage
-70 degree C
Stability: 12 months at -80 degree C
Other Notes
Small volumes of PTPN14 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
PTPN14 recombinant protein
Catalytic domain (a.a. 871-1188), MW=35kD, expressed in an E.coli expression system
Product Categories/Family for PTPN14 recombinant protein
Molecular Biology; MB-Enzymes, Phosphatase
Application Notes for PTPN14 recombinant protein
useful for the study of enzyme kinetics, regulation, and to dephosphorylate target substrates.
NCBI/Uniprot data below describe general gene information for PTPN14. It may not necessarily be applicable to this product.
NCBI Accession #
AAI04804.1
[Other Products]
UniProt Primary Accession #
Q15678
[Other Products]
UniProt Secondary Accession #
Q5VSI0[Other Products]
UniProt Related Accession #
Q15678[Other Products]
Molecular Weight
135,261 Da[Similar Products]
NCBI Official Full Name
Protein tyrosine phosphatase, non-receptor type 14
NCBI Official Synonym Full Names
protein tyrosine phosphatase, non-receptor type 14
NCBI Official Symbol
PTPN14??[Similar Products]
NCBI Official Synonym Symbols
PEZ; PTP36
??[Similar Products]
NCBI Protein Information
tyrosine-protein phosphatase non-receptor type 14; protein-tyrosine phosphatase pez; cytoskeletal-associated protein tyrosine phosphatase
UniProt Protein Name
Tyrosine-protein phosphatase non-receptor type 14
UniProt Synonym Protein Names
Protein-tyrosine phosphatase pez
Protein Family
Tyrosine-protein phosphatase non-receptor
UniProt Gene Name
PTPN14??[Similar Products]
UniProt Synonym Gene Names
PEZ; PTPD2??[Similar Products]
UniProt Entry Name
PTN14_HUMAN
NCBI Summary for PTPN14
The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains an N-terminal noncatalytic domain similar to that of band 4.1 superfamily cytoskeleton-associated proteins, which suggested the membrane or cytoskeleton localization of this protein. It appears to regulate lymphatic development in mammals, and a loss of function mutation has been found in a kindred with a lymphedema-choanal atresia. [provided by RefSeq, Sep 2010]
UniProt Comments for PTPN14
PTPN14: a non-receptor phospho-tyrosine protein phosphatase. Reported to be mutated in a small percentage of human cancers including colorectal cancers and a smaller fraction of lung, breast, and gastric cancers. May play a role in liver metastases and tumour invasion in pancreatic cancer. Contains 1 FERM domain.
Protein type: Motility/polarity/chemotaxis; EC 3.1.3.48; Protein phosphatase, tyrosine (non-receptor)
Chromosomal Location of Human Ortholog: 1q32.2
Cellular Component: nucleoplasm; cytoskeleton; cytoplasm; nucleus
Molecular Function: protein binding; transcription cofactor activity; protein tyrosine phosphatase activity; receptor tyrosine kinase binding
Biological Process: negative regulation of cell proliferation; transcription, DNA-dependent; regulation of transcription, DNA-dependent; lymphangiogenesis; protein amino acid dephosphorylation; regulation of protein export from nucleus
Disease: Choanal Atresia And Lymphedema
Research Articles on PTPN14
1. Data show that p130 Crk-associated substrate is a direct substrate of PTPN14 and that PTPN14 specifically regulates p130Cas phosphorylation at tyrosine residue 128 in colorectal cancer cells.
Precautions
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Disclaimer
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