GTPase NRas; Transforming protein N-Ras; NRAS; HRAS1

For Research Use Only. Not for use in diagnostic procedures.

Monoclonal

IgG1

822CT17.5.1

Mouse

This NRAS antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 70-101 amino acids from the N-terminal region of human NRAS.

Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.

Vial Concentration: 0.5 (lot specific)

Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Small volumes of anti-NRAS antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Ras proteins bind GDP/GTP and possess intrinsic GTPase activity.

Crown Antibodies; Cancer; Signal Transduction

Western Blot (WB), ELISA (EIA)

WB~~1:1000

NRAS Antibody (N-term) western blot analysis in A431 and rat C6 cell line lysates (35ug/lane).This demonstrates the NRAS antibody detected the NRAS protein (arrow).

21229

neuroblastoma RAS viral (v-ras) oncogene homolog

GTPase NRas

GTPase NRas

HRAS1??[Similar Products]

RASN_HUMAN

This is an N-ras oncogene encoding a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. This shuttling is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex. The encoded protein, which has intrinsic GTPase activity, is activated by a guanine nucleotide-exchange factor and inactivated by a GTPase activating protein. Mutations in this gene have been associated with somatic rectal cancer, follicular thyroid cancer, autoimmune lymphoproliferative syndrome, Noonan syndrome, and juvenile myelomonocytic leukemia. [provided by RefSeq, Jun 2011]

NRas: Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Interacts (active GTP-bound form preferentially) with RGS14. Alternate between an inactive form bound to GDP and an active form bound to GTP. Activated by a guanine nucleotide-exchange factor (GEF) and inactivated by a GTPase- activating protein (GAP). Belongs to the small GTPase superfamily. Ras family.

Protein type: G protein; Oncoprotein; G protein, monomeric; Motility/polarity/chemotaxis; G protein, monomeric, Ras

Chromosomal Location of Human Ortholog: 1p13.2

Cellular Component: Golgi membrane; membrane; plasma membrane

Molecular Function: GTP binding; protein complex binding

Biological Process: regulation of long-term neuronal synaptic plasticity; epidermal growth factor receptor signaling pathway; negative regulation of skeletal muscle development; axon guidance; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; activation of MAPKK activity; MAPKKK cascade; regulation of synaptic transmission, GABAergic; positive regulation of Rac protein signal transduction; negative regulation of cell differentiation; small GTPase mediated signal transduction; Ras protein signal transduction; positive regulation of cell proliferation; insulin receptor signaling pathway; striated muscle cell differentiation; visual learning; innate immune response; negative regulation of neuron apoptosis; actin cytoskeleton organization and biogenesis; blood coagulation; vascular endothelial growth factor receptor signaling pathway; leukocyte migration

Disease: Thyroid Carcinoma, Follicular; Ras-associated Autoimmune Leukoproliferative Disorder; Schimmelpenning-feuerstein-mims Syndrome; Noonan Syndrome 6; Melanocytic Nevus Syndrome, Congenital; Colorectal Cancer; Melanosis, Neurocutaneous; Nevus, Epidermal

Hall A. et al. Nucleic Acids Res. 13:5255-5268(1985).

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