Full Product Name
NRAS Monoclonal Antibody
Product Synonym Names
ALPS4; CMNS; N-ras; NCMS; NRAS1; NS6
Product Gene Name
anti-NRAS antibody
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P01111
Purity/Purification
Affinity Purification
Immunogen
A synthetic peptide of human NRAS
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Preparation and Storage
Store at 4 degree C. Avoid freeze/thaw cycles.
Other Notes
Small volumes of anti-NRAS antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-NRAS antibody
This is an N-ras oncogene encoding a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. This shuttling is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex. The encoded protein, which has intrinsic GTPase activity, is activated by a guanine nucleotide-exchange factor and inactivated by a GTPase activating protein. Mutations in this gene have been associated with somatic rectal cancer, follicular thyroid cancer, autoimmune lymphoproliferative syndrome, Noonan syndrome, and juvenile myelomonocytic leukemia.
Product Categories/Family for anti-NRAS antibody
Primary antibody
Applications Tested/Suitable for anti-NRAS antibody
Western Blot (WB)
Application Notes for anti-NRAS antibody
WB: 1:200 - 1:500
Western Blot (WB) of anti-NRAS antibody
Western blot analysis of extracts of various cell lines, using NRAS antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (MBS128212) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
NCBI/Uniprot data below describe general gene information for NRAS . It may not necessarily be applicable to this product.
NCBI Accession #
P01111.1
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UniProt Primary Accession #
P01111
[Other Products]
UniProt Secondary Accession #
Q14971; Q15104; Q15282[Other Products]
UniProt Related Accession #
P01111[Other Products]
NCBI Official Full Name
GTPase NRas
NCBI Official Synonym Full Names
NRAS proto-oncogene, GTPase
NCBI Official Symbol
NRAS??[Similar Products]
NCBI Official Synonym Symbols
NS6; CMNS; NCMS; ALPS4; N-ras; NRAS1
??[Similar Products]
NCBI Protein Information
GTPase NRas
UniProt Protein Name
GTPase NRas
UniProt Synonym Protein Names
Transforming protein N-Ras
UniProt Gene Name
NRAS??[Similar Products]
UniProt Synonym Gene Names
HRAS1??[Similar Products]
NCBI Summary for NRAS
This is an N-ras oncogene encoding a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. This shuttling is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex. The encoded protein, which has intrinsic GTPase activity, is activated by a guanine nucleotide-exchange factor and inactivated by a GTPase activating protein. Mutations in this gene have been associated with somatic rectal cancer, follicular thyroid cancer, autoimmune lymphoproliferative syndrome, Noonan syndrome, and juvenile myelomonocytic leukemia. [provided by RefSeq, Jun 2011]
UniProt Comments for NRAS
NRas: Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Interacts (active GTP-bound form preferentially) with RGS14. Alternate between an inactive form bound to GDP and an active form bound to GTP. Activated by a guanine nucleotide-exchange factor (GEF) and inactivated by a GTPase- activating protein (GAP). Belongs to the small GTPase superfamily. Ras family.
Protein type: G protein; G protein, monomeric; G protein, monomeric, Ras; Motility/polarity/chemotaxis; Oncoprotein
Chromosomal Location of Human Ortholog: 1p13.2
Cellular Component: Golgi apparatus; membrane; plasma membrane
Molecular Function: protein complex binding
Biological Process: axon guidance; epidermal growth factor receptor signaling pathway; leukocyte migration; MAPKKK cascade; neutrophil degranulation; Ras protein signal transduction; stimulatory C-type lectin receptor signaling pathway
Disease: Colorectal Cancer; Melanocytic Nevus Syndrome, Congenital; Melanosis, Neurocutaneous; Nevus, Epidermal; Noonan Syndrome 6; Ras-associated Autoimmune Leukoproliferative Disorder; Schimmelpenning-feuerstein-mims Syndrome; Thyroid Carcinoma, Follicular
Research Articles on NRAS
1. review focuses on the genes, which are frequently mutated in various cancers and are known to be important in the advance and progression of colorectal cancer and melanoma, namely KRAS, NRAS and BRAF
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Disclaimer
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