N-RAS; NS6; ALPS4; N-ras; NRAS1; HRAS1; GTPase NRas; Transforming protein N-Ras; neuroblastoma RAS viral (v-ras) oncogene homolog

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

IgG

Rabbit

N-RAS antibody is predicted to not cross-react with H-RAS

N-RAS Antibody is affinity chromatography purified via peptide column.

Liquid

1 mg/mL (lot specific)

N-RAS antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year.

Small volumes of anti-NRAS antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

N-RAS Antibody: Activating mutations and overexpression of classical Ras subfamily members (N-RAS, H-RAS, and K-RAS) have been widely investigated as key events in the development of human cancers. The N-RAS protein shuttles between the Golgi apparatus and the plasma membrane. This shuttling is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex. N-RAS, which has intrinsic GTPase activity, is activated by a guanine nucleotide-exchange factor and inactivated by a GTPase activating protein. Mutations in this gene have been associated with somatic rectal cancer, follicular thyroid cancer, autoimmune lymphoproliferative syndrome, Noonan syndrome, and juvenile myelomonocytic leukemia.

ELISA (EIA), Western Blot (WB)

N-RAS antibody can be used for detection of N-RAS by Western blot at 0.5 - 1 mug/mL.

Western blot analysis of NRAS in A431 cell lysate with NRAS antibody at (A) 1 and (B) 2 μg/mL.

Predicted: 21

Observed: 20

neuroblastoma RAS viral (v-ras) oncogene homolog

GTPase NRas; N-ras protein part 4; transforming protein N-Ras; v-ras neuroblastoma RAS viral oncogene homolog

GTPase NRas

HRAS1??[Similar Products]

RASN_HUMAN

This is an N-ras oncogene encoding a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. This shuttling is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex. The encoded protein, which has intrinsic GTPase activity, is activated by a guanine nucleotide-exchange factor and inactivated by a GTPase activating protein. Mutations in this gene have been associated with somatic rectal cancer, follicular thyroid cancer, autoimmune lymphoproliferative syndrome, Noonan syndrome, and juvenile myelomonocytic leukemia. [provided by RefSeq, Jun 2011]

NRas: Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Interacts (active GTP-bound form preferentially) with RGS14. Alternate between an inactive form bound to GDP and an active form bound to GTP. Activated by a guanine nucleotide-exchange factor (GEF) and inactivated by a GTPase- activating protein (GAP). Belongs to the small GTPase superfamily. Ras family.

Protein type: G protein, monomeric, Ras; G protein; Oncoprotein; Motility/polarity/chemotaxis; G protein, monomeric

Chromosomal Location of Human Ortholog: 1p13.2

Cellular Component: Golgi membrane; membrane; plasma membrane

Molecular Function: GTP binding; protein complex binding

Biological Process: regulation of long-term neuronal synaptic plasticity; epidermal growth factor receptor signaling pathway; negative regulation of skeletal muscle development; axon guidance; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; activation of MAPKK activity; MAPKKK cascade; regulation of synaptic transmission, GABAergic; positive regulation of Rac protein signal transduction; negative regulation of cell differentiation; small GTPase mediated signal transduction; positive regulation of cell proliferation; Ras protein signal transduction; insulin receptor signaling pathway; visual learning; striated muscle cell differentiation; innate immune response; negative regulation of neuron apoptosis; blood coagulation; actin cytoskeleton organization and biogenesis; vascular endothelial growth factor receptor signaling pathway; leukocyte migration

Disease: Thyroid Carcinoma, Follicular; Ras-associated Autoimmune Leukoproliferative Disorder; Noonan Syndrome 6; Schimmelpenning-feuerstein-mims Syndrome; Melanocytic Nevus Syndrome, Congenital; Colorectal Cancer; Melanosis, Neurocutaneous; Nevus, Epidermal

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