Product Name
N-RAS (NRAS), Blocking Peptide
Full Product Name
N-RAS Peptide
Product Synonym Names
NS6; ALPS4; N-ras; NRAS1; HRAS1; GTPase NRas; Transforming protein N-Ras; neuroblastoma RAS viral (v-ras) oncogene homolog
Product Gene Name
NRAS blocking peptide
[Similar Products]
N-RAS peptide (MBS153898) is used for blocking the activity of N-RAS antibody (MBS153718)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P01111
Concentration
200 ug/mL (lot specific)
Buffer
PBS pH 7.2 (10 mM NaH2PO4, 10 mM Na2HPO4, 130 mM NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide
Location
15 amino acids near the carboxy-terminus of human N-RAS.
Preparation and Storage
Store N-RAS peptide at -20 degree C, stable for one year.
Other Notes
Small volumes of NRAS blocking peptide vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for NRAS blocking peptide
Blocking (BL)
Application Notes for NRAS blocking peptide
N-RAS peptide is used for blocking the activity of N-RAS antibody. It usually blocks the antibody activity completely in Western blot by incubating the peptide with equal volume of antibody for 30 min at 37 degree C.
NCBI/Uniprot data below describe general gene information for NRAS. It may not necessarily be applicable to this product.
NCBI Accession #
NP_002515
[Other Products]
NCBI GenBank Nucleotide #
NM_002524.4
[Other Products]
UniProt Primary Accession #
P01111
[Other Products]
UniProt Secondary Accession #
Q14971; Q15104; Q15282[Other Products]
UniProt Related Accession #
P01111[Other Products]
Molecular Weight
21,229 Da
NCBI Official Full Name
GTPase NRas
NCBI Official Synonym Full Names
neuroblastoma RAS viral (v-ras) oncogene homolog
NCBI Official Symbol
NRAS??[Similar Products]
NCBI Official Synonym Symbols
NS6; CMNS; NCMS; ALPS4; N-ras; NRAS1
??[Similar Products]
NCBI Protein Information
GTPase NRas; N-ras protein part 4; transforming protein N-Ras; v-ras neuroblastoma RAS viral oncogene homolog
UniProt Protein Name
GTPase NRas
UniProt Synonym Protein Names
Transforming protein N-Ras
UniProt Gene Name
NRAS??[Similar Products]
UniProt Synonym Gene Names
HRAS1??[Similar Products]
UniProt Entry Name
RASN_HUMAN
NCBI Summary for NRAS
This is an N-ras oncogene encoding a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. This shuttling is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex. The encoded protein, which has intrinsic GTPase activity, is activated by a guanine nucleotide-exchange factor and inactivated by a GTPase activating protein. Mutations in this gene have been associated with somatic rectal cancer, follicular thyroid cancer, autoimmune lymphoproliferative syndrome, Noonan syndrome, and juvenile myelomonocytic leukemia. [provided by RefSeq, Jun 2011]
UniProt Comments for NRAS
NRas: Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Interacts (active GTP-bound form preferentially) with RGS14. Alternate between an inactive form bound to GDP and an active form bound to GTP. Activated by a guanine nucleotide-exchange factor (GEF) and inactivated by a GTPase- activating protein (GAP). Belongs to the small GTPase superfamily. Ras family.
Protein type: G protein; Oncoprotein; G protein, monomeric, Ras; Motility/polarity/chemotaxis; G protein, monomeric
Chromosomal Location of Human Ortholog: 1p13.2
Cellular Component: Golgi membrane; membrane; plasma membrane
Molecular Function: GTP binding; protein complex binding
Biological Process: epidermal growth factor receptor signaling pathway; regulation of long-term neuronal synaptic plasticity; axon guidance; negative regulation of skeletal muscle development; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; activation of MAPKK activity; MAPKKK cascade; regulation of synaptic transmission, GABAergic; positive regulation of Rac protein signal transduction; negative regulation of cell differentiation; small GTPase mediated signal transduction; Ras protein signal transduction; positive regulation of cell proliferation; insulin receptor signaling pathway; striated muscle cell differentiation; innate immune response; visual learning; negative regulation of neuron apoptosis; actin cytoskeleton organization and biogenesis; vascular endothelial growth factor receptor signaling pathway; blood coagulation; leukocyte migration
Disease: Thyroid Carcinoma, Follicular; Ras-associated Autoimmune Leukoproliferative Disorder; Noonan Syndrome 6; Schimmelpenning-feuerstein-mims Syndrome; Melanocytic Nevus Syndrome, Congenital; Colorectal Cancer; Melanosis, Neurocutaneous; Nevus, Epidermal
Research Articles on NRAS
1. Mutations of N-ras are present in multiple congenital neavi. A nonsense mutation afects the codon 61 N-ras.
Precautions
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Disclaimer
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