ARHGAP26; GRAF; Rho GTPase activating protein 26; KIAA0621; FLJ42530; OPHN1L; OPHN1L1; GTPase regulator associated with FAK; GTPase regulator associated with focal adhesion kinase pp125(FAK); GTPase regulator associated with the focal adhesion kinase pp1; ARHGAP26 antibody; GRAF antibody; Rho GTPase activating protein 26 antibody; KIAA0621 antibody; FLJ42530 antibody; OPHN1L antibody; OPHN1L1 antibody; GTPase regulator associated with FAK antibody; GTPase regulator associated with focal adhesion kinase pp125(FAK) antibody; GTPase regulator associated with the focal adhesion kinase pp125 antibody; GRAF / ARHGAP26

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Goat

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100ug specific antibody in 200ul (lot specific)

Aliquot and store at -20 degree C. Minimize freezing and thawing.

Manufactured in an ISO 9001:2008 Certified Laboratory.

Small volumes of anti-ARHGAP26 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Peptide ELISA (EIA), Western Blot (WB)

Peptide ELISA: Antibody detection limit dilution 1: 64000.
Western Blot: Approx 90kDa band observed in Human Heart lysates (calculated MW of 92.2kDa according to NP_055886.1). Recommended concentration: 0.3-1ug/ml. An additional strong band of unknown identity was also consistently observed at 16kDa. This band was successfully blocked by incubation with the immunising peptide.

(0.3ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.

86,165 Da

Rho GTPase activating protein 26

rho GTPase-activating protein 26

Rho GTPase-activating protein 26

GRAF; KIAA0621; OPHN1L??[Similar Products]

RHG26_HUMAN

Interaction of a cell with the extracellular matrix triggers integrin cell surface receptors to begin signaling cascades that regulate the organization of the actin-cytoskeleton. One of the proteins involved in these cascades is focal adhesion kinase. The protein encoded by this gene is a GTPase activating protein that binds to focal adhesion kinase and mediates the activity of the GTP binding proteins RhoA and Cdc42. Defects in this gene are a cause of juvenile myelomonocytic leukemia (JMML). Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010]

ARHGAP26: GTPase-activating protein for RHOA and CDC42. Interacts with NYAP1, NYAP2 and MYO16. Binds to the C-terminus of PTK2/FAK1. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Oncoprotein; GAPs; GAPs, Rac/Rho

Chromosomal Location of Human Ortholog: 5q31

Cellular Component: cytoskeleton; cytosol; focal adhesion

Molecular Function: GTPase activator activity; phospholipid binding; protein binding

Biological Process: actin cytoskeleton organization and biogenesis; nervous system development; positive regulation of GTPase activity; regulation of small GTPase mediated signal transduction; signal transduction

Disease: Juvenile Myelomonocytic Leukemia

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