Full Product Name
RPL11 Antibody Pair
Product Synonym Names
60S ribosomal protein L11; CLL-associated antigen KW-12
Product Gene Name
RPL11 antibody pair
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Isotype
Capture: IgG
Detection: IgG
Host
Capture: Rabbit
Detection: Rabbit
Species Reactivity
Human, Rat, Mouse, Guinea Pig, Bovine, Horse, Pig, Dog, Chicken, Goat
Concentration
The concentrations and volumes of the antibodies may vary and be lot specific. Details will be on the vial labels. (lot specific)
Conjugation
Capture: Non-conjugated; Detection: Biotin conjugated
Recommended Standard
SF-268 cell; Hela cell
(Standards are recommendations only. Compatibility of the standards in the user developed application needs to be established and confirmed by the researcher.
Storage Buffer
Capture: 50% Glycerol, 0.01M PBS, PH 7.4
Detection: 50% Glycerol, 0.01M PBS, PH 7.4
Other Note
Immunogen information is company proprietary information. Antibodies are sold as a pair and not available separately.
Preparation and Storage
Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze/thaw cycles. When stored properly, products should be stable for at least 5 months.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Other Notes
Small volumes of RPL11 antibody pair vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for RPL11 antibody pair
Sandwich ELISA (EIA)
Application Notes for RPL11 antibody pair
We recommend using the capture antibody at a concentration of 0.5ug/ml and the detection antibody at a concentration of 0.34ug/ml.
NCBI/Uniprot data below describe general gene information for RPL11. It may not necessarily be applicable to this product.
NCBI Accession #
AKI70806.1
[Other Products]
UniProt Secondary Accession #
P25121; P39026; Q8TDH2; Q9Y674[Other Products]
UniProt Related Accession #
P62913[Other Products]
Molecular Weight
20,124 Da
NCBI Official Full Name
RPL11, partial
NCBI Official Synonym Full Names
ribosomal protein L11
NCBI Official Symbol
RPL11??[Similar Products]
NCBI Official Synonym Symbols
L11; DBA7; GIG34
??[Similar Products]
NCBI Protein Information
60S ribosomal protein L11
UniProt Protein Name
60S ribosomal protein L11
UniProt Synonym Protein Names
CLL-associated antigen KW-12
Protein Family
50S ribosomal protein
UniProt Gene Name
RPL11??[Similar Products]
UniProt Entry Name
RL11_HUMAN
NCBI Summary for RPL11
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L5P family of ribosomal proteins. It is located in the cytoplasm. The protein probably associates with the 5S rRNA. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Dec 2010]
UniProt Comments for RPL11
RPL11: Binds to 5S ribosomal RNA. Required for rRNA maturation and formation of the 60S ribosomal subunits. Promotes nucleolar location of PML. Defects in RPL11 are the cause of Diamond-Blackfan anemia type 7 (DBA7). DBA7 is a form of Diamond-Blackfan anemia, a congenital non-regenerative hypoplastic anemia that usually presents early in infancy. Diamond-Blackfan anemia is characterized by a moderate to severe macrocytic anemia, erythroblastopenia, and an increased risk of malignancy. 30 to 40% of Diamond-Blackfan anemia patients present with short stature and congenital anomalies, the most frequent being craniofacial (Pierre-Robin syndrome and cleft palate), thumb and urogenital anomalies. Belongs to the ribosomal protein L5P family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Ribosomal; Motility/polarity/chemotaxis; Translation; Nucleolus
Chromosomal Location of Human Ortholog: 1p36.1-p35
Cellular Component: cytoplasm; cytosol; membrane; nucleolus
Molecular Function: protein binding; RNA binding; rRNA binding; structural constituent of ribosome
Biological Process: mRNA catabolic process, nonsense-mediated decay; protein targeting; ribosomal large subunit assembly and maintenance; ribosomal large subunit biogenesis and assembly; rRNA processing; SRP-dependent cotranslational protein targeting to membrane; translation; translational initiation; viral transcription
Disease: Diamond-blackfan Anemia 7
Research Articles on RPL11
1. Data suggest 5S ribosomal RNA is a direct target of miR-150 and miR-383 in esophageal squamous cell carcinoma (ESCC); overexpression of miR-150 and miR-383 inhibits ESCC cell proliferation in vitro and in vivo and intensifies RPL11/c-Myc interaction.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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