Product Name
RPL11, siRNA
Full Product Name
RPL11 siRNA (Human)
Product Synonym Names
60S ribosomal protein L11; CLL-associated antigen KW-12
Product Gene Name
RPL11 sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P62913
Specificity
RPL11 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human RPL11 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of RPL11 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
RPL11 sirna
siRNA to inhibit RPL11 expression using RNA interference
Applications Tested/Suitable for RPL11 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for RPL11. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000966.2
[Other Products]
NCBI GenBank Nucleotide #
NM_000975.3
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UniProt Primary Accession #
P62913
[Other Products]
UniProt Secondary Accession #
P25121; P39026; Q8TDH2; Q9Y674[Other Products]
UniProt Related Accession #
P62913[Other Products]
Molecular Weight
20,124 Da
NCBI Official Full Name
60S ribosomal protein L11 isoform 1
NCBI Official Synonym Full Names
ribosomal protein L11
NCBI Official Symbol
RPL11??[Similar Products]
NCBI Official Synonym Symbols
L11; DBA7; GIG34
??[Similar Products]
NCBI Protein Information
60S ribosomal protein L11
UniProt Protein Name
60S ribosomal protein L11
UniProt Synonym Protein Names
CLL-associated antigen KW-12
Protein Family
50S ribosomal protein
UniProt Gene Name
RPL11??[Similar Products]
UniProt Entry Name
RL11_HUMAN
NCBI Summary for RPL11
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L5P family of ribosomal proteins. It is located in the cytoplasm. The protein probably associates with the 5S rRNA. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Dec 2010]
UniProt Comments for RPL11
RPL11: Binds to 5S ribosomal RNA. Required for rRNA maturation and formation of the 60S ribosomal subunits. Promotes nucleolar location of PML. Defects in RPL11 are the cause of Diamond-Blackfan anemia type 7 (DBA7). DBA7 is a form of Diamond-Blackfan anemia, a congenital non-regenerative hypoplastic anemia that usually presents early in infancy. Diamond-Blackfan anemia is characterized by a moderate to severe macrocytic anemia, erythroblastopenia, and an increased risk of malignancy. 30 to 40% of Diamond-Blackfan anemia patients present with short stature and congenital anomalies, the most frequent being craniofacial (Pierre-Robin syndrome and cleft palate), thumb and urogenital anomalies. Belongs to the ribosomal protein L5P family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Motility/polarity/chemotaxis; Nucleolus; Ribosomal; Translation
Chromosomal Location of Human Ortholog: 1p36.1-p35
Cellular Component: membrane; cytoplasm; nucleolus; cytosol
Molecular Function: rRNA binding; protein binding; structural constituent of ribosome; RNA binding
Biological Process: SRP-dependent cotranslational protein targeting to membrane; translation; viral reproduction; viral infectious cycle; ribosomal large subunit biogenesis and assembly; translational termination; translational elongation; cellular protein metabolic process; mRNA catabolic process, nonsense-mediated decay; translational initiation; viral transcription; gene expression; rRNA processing; protein targeting
Disease: Diamond-blackfan Anemia 7
Research Articles on RPL11
1. Data indicate that ribosomal protein L11 (RPL11)-expressing cells proliferated more rapidly than the ribosomal protein L11 (RPL11)-expressing cells.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
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