Product Name
SH3BP2, siRNA
Full Product Name
SH3BP2 siRNA (Human)
Product Synonym Names
3BP2; SH3 domain-binding protein 2; 3BP-2
Product Gene Name
SH3BP2 sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P78314
Specificity
SH3BP2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human SH3BP2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of SH3BP2 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
SH3BP2 sirna
siRNA to inhibit SH3BP2 expression using RNA interference
Applications Tested/Suitable for SH3BP2 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for SH3BP2. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001116153.1
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NCBI GenBank Nucleotide #
NM_001122681.1
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UniProt Primary Accession #
P78314
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UniProt Secondary Accession #
O00500; O15373; P78315; A6NNC2; B2R5R6; B4DT04; D3DVR0; D6R919[Other Products]
UniProt Related Accession #
P78314[Other Products]
Molecular Weight
68,129 Da
NCBI Official Full Name
SH3 domain-binding protein 2 isoform a
NCBI Official Synonym Full Names
SH3-domain binding protein 2
NCBI Official Symbol
SH3BP2??[Similar Products]
NCBI Official Synonym Symbols
3BP2; CRBM; CRPM; 3BP-2; RES4-23
??[Similar Products]
NCBI Protein Information
SH3 domain-binding protein 2
UniProt Protein Name
SH3 domain-binding protein 2
Protein Family
SH3 domain-binding protein
UniProt Gene Name
SH3BP2??[Similar Products]
UniProt Synonym Gene Names
3BP2; 3BP-2??[Similar Products]
UniProt Entry Name
3BP2_HUMAN
NCBI Summary for SH3BP2
The protein encoded by this gene has an N-terminal pleckstrin homology (PH) domain, an SH3-binding proline-rich region, and a C-terminal SH2 domain. The protein binds to the SH3 domains of several proteins including the ABL1 and SYK protein tyrosine kinases , and functions as a cytoplasmic adaptor protein to positively regulate transcriptional activity in T, natural killer (NK), and basophilic cells. Mutations in this gene result in cherubism. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]
UniProt Comments for SH3BP2
SH3BP2: an adaptor protein that binds differentially to the SH3 domains of certain proteins. Links the hemopoietic tyrosine kinase Fes to the cytoplasmic membrane in a phosphorylation dependent manner. Regulates FC-epsilon RI -mediated degranulation in rat basophilic leukemia RBL-2H3 cells. Expressed in a variety of tissues including lung, liver, skeletal muscle, kidney and pancreas. Defects in 3BP2 are the cause of cherubism, an autosomal dominant inherited syndrome. Two differentially spliced isoforms have been described.
Protein type: Adaptor/scaffold
Chromosomal Location of Human Ortholog: 4p16.3
Molecular Function: protein binding; SH3/SH2 adaptor activity; SH3 domain binding
Biological Process: positive regulation of signal transduction; signal transduction
Disease: Cherubism
Research Articles on SH3BP2
1. The adaptor 3BP2 is required for KIT receptor expression and human mast cell survival.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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