Product Name
CIRH1A, siRNA
Full Product Name
CIRH1A siRNA (Mouse)
Product Synonym Names
KIAA1988; TEX292; Cirhin; Testis-expressed gene 292 protein
Product Gene Name
CIRH1A sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q8R2N2
Specificity
CIRH1A siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse CIRH1A gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of CIRH1A sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
CIRH1A sirna
siRNA to inhibit CIRH1A expression using RNA interference
Applications Tested/Suitable for CIRH1A sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for CIRH1A. It may not necessarily be applicable to this product.
NCBI Accession #
NP_035704.2
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NCBI GenBank Nucleotide #
NM_011574.2
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UniProt Primary Accession #
Q8R2N2
[Other Products]
UniProt Secondary Accession #
Q62306; Q62307; Q69Z48; Q6NVG9; Q8VEL5[Other Products]
UniProt Related Accession #
Q8R2N2[Other Products]
Molecular Weight
76,909 Da
NCBI Official Full Name
cirhin
NCBI Official Synonym Full Names
cirrhosis, autosomal recessive 1A (human)
NCBI Official Symbol
Cirh1a??[Similar Products]
NCBI Official Synonym Symbols
Naic; Cirhin; Tex292; Teg-292
??[Similar Products]
NCBI Protein Information
cirhin
UniProt Protein Name
Cirhin
UniProt Synonym Protein Names
Testis-expressed gene 292 protein
UniProt Gene Name
Cirh1a??[Similar Products]
UniProt Synonym Gene Names
Kiaa1988; Tex292??[Similar Products]
UniProt Entry Name
CIR1A_MOUSE
UniProt Comments for CIRH1A
CIRH1A: May be a transcriptional regulator. Acts as a positive regulator of HIVEP1 which specifically binds to the DNA sequence 5'-GGGACTTTCC-3' found in enhancer elements of numerous viral promoters such as those of HIV-1, SV40, or CMV. Defects in CIRH1A are the cause of North American Indian childhood cirrhosis (NAIC). NAIC is a severe autosomal recessive intrahepatic cholestasis, originally described in Ojibway-Cree children from northwestern Quebec. NAIC typically presents with transient neonatal jaundice, in a child who is otherwise healthy, and progresses to biliary cirrhosis and portal hypertension. Biochemical and histopathological features suggest involvement of the bile ducts rather than of the bile canaliculi. They include elevated gamma glutamyltransferase and alkaline phosphatase levels, and, typically, marked fibrosis around bile ducts. Clinically, NAIC is distinct from other nonsyndromic familial cholestases because of its marked cholangiopathic features and severe degree of fibrosis on liver histology. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Unknown function; Nucleolus
Cellular Component: nucleolus; nucleus
Biological Process: transcription, DNA-dependent; regulation of transcription, DNA-dependent; rRNA processing; maturation of SSU-rRNA
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
