DAPAT; DAP-AT; DHAPAT

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

IgG

Rabbit

Affinity Purification

1mg/ml (lot specific)

Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Small volumes of anti-GNPAT antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

This gene encodes an enzyme located in the peroxisomal membrane which is essential to the synthesis of ether phospholipids. Mutations in this gene are associated with rhizomelic chondrodysplasia punctata.

Polyclonal

Western Blot (WB), Immunofluorescence (IF)

WB: 1:500 - 1:2000
IF: 1:50 - 1:200

Western blot analysis of extracts of various cell lines, using GNPAT antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.

680

glyceronephosphate O-acyltransferase

dihydroxyacetone phosphate acyltransferase; DHAP-AT; glycerone-phosphate O-acyltransferase; acyl-CoA:dihydroxyacetonephosphateacyltransferase

Dihydroxyacetone phosphate acyltransferase

DAPAT; DHAPAT; DAP-AT; DHAP-AT??[Similar Products]

GNPAT_HUMAN

This gene encodes an enzyme located in the peroxisomal membrane which is essential to the synthesis of ether phospholipids. Mutations in this gene are associated with rhizomelic chondrodysplasia punctata. [provided by RefSeq, Jul 2008]

GNPAT: Defects in GNPAT are the cause of rhizomelic chondrodysplasia punctata type 2 (RCDP2). RDCP2 is characterized by rhizomelic shortening of femur and humerus, vertebral disorders, cataract, cutaneous lesions and severe mental retardation. Belongs to the GPAT/DAPAT family.

Protein type: EC 2.3.1.42; Lipid Metabolism - glycerophospholipid; Transferase

Chromosomal Location of Human Ortholog: 1q42

Cellular Component: peroxisomal membrane; peroxisomal matrix; mitochondrion; membrane; peroxisome

Molecular Function: glycerone-phosphate O-acyltransferase activity; palmitoyl-CoA hydrolase activity; receptor binding

Biological Process: response to drug; response to starvation; synaptogenesis; phospholipid metabolic process; glycerophospholipid biosynthetic process; cerebellum morphogenesis; phosphatidic acid biosynthetic process; cellular lipid metabolic process; response to nutrient; ether lipid biosynthetic process; paranodal junction assembly

Disease: Rhizomelic Chondrodysplasia Punctata, Type 2

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