LDM; CP51; CYP51; CYPL1; P450L1; P450-14DM

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

IgG

Rabbit

Affinity Purification

1mg/ml (lot specific)

Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Small volumes of anti-CYP51A1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum protein participates in the synthesis of cholesterol by catalyzing the removal of the 14alpha-methyl group from lanosterol. Homologous genes are found in all three eukaryotic phyla, fungi, plants, and animals, suggesting that this is one of the oldest cytochrome P450 genes. Two transcript variants encoding different isoforms have been found for this gene.

Polyclonal

Western Blot (WB), Immunohistochemistry (IHC)

WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:20 - 1:100

Western blot analysis of extracts of various cell lines, using CYP51A1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.

Immunohistochemistry of paraffin-embedded mouse kidney using CYP51A1 Antibody at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat lung using CYP51A1 Antibody at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human liver using CYP51A1 Antibody at dilution of 1:100 (40x lens).

503

cytochrome P450, family 51, subfamily A, polypeptide 1

lanosterol 14-alpha demethylase; CYPLI; cytochrome P450LI; cytochrome P45014DM; cytochrome P450 51A1; cytochrome P450-14DM; lanosterol 14-alpha-demethylase; cytochrome P450, 51 (lanosterol 14-alpha-demethylase)

Lanosterol 14-alpha demethylase

CYP51; LDM; Cytochrome P45014DM??[Similar Products]

CP51A_HUMAN

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum protein participates in the synthesis of cholesterol by catalyzing the removal of the 14alpha-methyl group from lanosterol. Homologous genes are found in all three eukaryotic phyla, fungi, plants, and animals, suggesting that this is one of the oldest cytochrome P450 genes. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]

CYP51A1: Catalyzes C14-demethylation of lanosterol; it transforms lanosterol into 4,4'-dimethyl cholesta-8,14,24-triene-3-beta-ol. Belongs to the cytochrome P450 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Oxidoreductase; EC 1.14.13.70; Membrane protein, integral; Lipid Metabolism - steroid biosynthesis

Chromosomal Location of Human Ortholog: 7q21.2

Cellular Component: endoplasmic reticulum membrane; membrane; endoplasmic reticulum; integral to membrane

Molecular Function: sterol 14-demethylase activity; iron ion binding; heme binding

Biological Process: xenobiotic metabolic process; cholesterol biosynthetic process; steroid biosynthetic process; sterol metabolic process; cholesterol biosynthetic process via 24,25-dihydrolanosterol

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