For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

IgG

Rabbit

The antibody is a rabbit polyclonal antibody raised against CARD9. It has beenselected for its ability to recognize CARD9 in immunohistochemical staining andwestern blotting.

Affinity Chromatography

Liquid

200ug/ml (lot specific)

Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.

Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customera??s specifications, please inquire.

Small volumes of anti-CARD9 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Knock-Out Validation Antibody

Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)

Western blotting: 0.2-2ug/mL;1:250-2500
Immunohistochemistry: 5-20ug/mL;1:25-100
Immunocytochemistry: 5-20ug/mL;1:25-100
Optimal working dilutions must be determined by end user.

Western Blot: Sample: Human HepG2 cell lysate; Primary Ab: 3ug/ml Rabbit Anti-Human CARD9 Antibody Second Ab: 0.2ug/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody

Western Blot: Sample: Recombinant protein.

DAB staining on fromalin fixed paraffin- embedded Kidney tissue)

DAB staining on IHC-P; Samples: Human Glioma Tissue)

Knockout Validation: Lane 1: Wild-type PC3 cell lysate; Lane 2: CARD9 knockout PC3 cell lysate; Predicted MW: 62,56,43kd Observed MW: 50kd Primary Ab: 3ug/ml Rabbit Anti-Human CARD9 Antibody Second Ab: 0.2ug/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047)

42,953 Da

caspase recruitment domain family member 9

caspase recruitment domain-containing protein 9

Caspase recruitment domain-containing protein 9

hCARD9??[Similar Products]

The protein encoded by this gene is a member of the CARD protein family, which is defined by the presence of a characteristic caspase-associated recruitment domain (CARD). CARD is a protein interaction domain known to participate in activation or suppression of CARD containing members of the caspase family, and thus plays an important regulatory role in cell apoptosis. This protein was identified by its selective association with the CARD domain of BCL10, a postive regulator of apoptosis and NF-kappaB activation, and is thought to function as a molecular scaffold for the assembly of a BCL10 signaling complex that activates NF-kappaB. Several alternatively spliced transcript variants have been observed, but their full-length nature is not clearly defined. [provided by RefSeq, Jul 2008]

CARD9: Activates NF-kappa-B via BCL10. Defects in CARD9 are the cause of familial candidiasis type 2 (CANDF2). Chronic mucocutaneous candidiasis is characterized by impaired clearance of fungal infections and results in colonization and infections of the mucosa or skin, predominantly with Candida albicans. CANDF2 is an autosomal recessive chronic mucocutaneous candidiasis. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 9q34.3

Cellular Component: cytoplasm; cytosol; plasma membrane

Molecular Function: CARD domain binding; protein binding; protein homodimerization activity

Biological Process: defense response to Gram-positive bacterium; defense response to virus; I-kappaB kinase/NF-kappaB signaling; innate immune response; positive regulation of I-kappaB kinase/NF-kappaB signaling; positive regulation of interleukin-6 production; positive regulation of JNK cascade; positive regulation of stress-activated MAPK cascade; positive regulation of tumor necrosis factor production; regulation of apoptosis; regulation of interleukin-2 biosynthetic process; regulation of interleukin-6 biosynthetic process; regulation of tumor necrosis factor biosynthetic process; response to drug; response to exogenous dsRNA; response to fungus; response to muramyl dipeptide; response to peptidoglycan; stimulatory C-type lectin receptor signaling pathway

Disease: Candidiasis, Familial, 2

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