Product Name
AITRL (TNFSF18), Recombinant Protein
Full Product Name
AITRL, Human Recombinant
Product Synonym Names
Activation-induced TNFR member Ligand; TNFSF18; GITRL; TL-6
Product Gene Name
TNFSF18 recombinant protein
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence
METAKEPCMA KFGPLPSKWQ MASSEPPCVN KVSDWKLEIL QNGLYLIYGQ VAPNANYNDV APFEVRLYKN KDMIQTLTNK SKIQNVGGTY ELHVGDTIDL IFNSEHQVLK NNTYWGIILL ANPQFIS
3D Structure
ModBase 3D Structure for Q9UNG2
Purity/Purification
>=98%
Form/Format
Sterile filtered through a 0.2 micron filter. Lyophilized from 10 mM Tris, pH 8.0.
Appearance: Lyophilized powder
Solubility/Reconstitution Instructions
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20 degree C to -80 degree C.
Endotoxin Level
< 0.1 ng/mug of protein (<1EU/mug).
Activity (Specifications/test method)
Determined by its ability to stimulate IL-8 production by human PBMC using a concentration range of 1.0-10.0 ng/ml. Note: Results may vary with PBMC donors.
Biological Activity
Determined by its ability to stimulate IL-8 production by human PBMC using a concentration range of 1.0-10.0 ng/ml. Note: Results may vary with PBMC donors.
Handling
Centrifuge the vial prior to opening.
Preparation and Storage
At -20 degree C
Shelf Life: 12 months
Other Notes
Small volumes of TNFSF18 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
TNFSF18 recombinant protein
Background: AITRL, a member of the TNF superfamily, is expressed in endothelial cells, and signals through the AITR receptor. AITRL regulates T-cell proliferation and survival, and effectuates the interaction between T lymphocytes and endothelial cells. The AITRL gene codes for a type II transmembrane protein comprised of 177 amino acids, including a 28 amino acid cytoplasmic region, a 21 amino acid transmembrane domain and a 128 amino acid extracellular domain. Recombinant human soluble AITRL is a 14.4 kDa protein, containing 127 amino acid residues corresponding to the extracellular domain of AITRL.
Product Categories/Family for TNFSF18 recombinant protein
Cytokines, Growth Factors & Hormones; TNFsCytokines, Growth Factors & Hormones; Cytokines & Growth Factors (A-Z)Proteins and Enzymes; Cytokines, Growth Factors & Hormones; TNFsProteins and Enzymes; Cytokines, Growth Factors & Hormones; Cytokines & Growth
Applications Tested/Suitable for TNFSF18 recombinant protein
SDS-PAGE; HPLC
NCBI/Uniprot data below describe general gene information for TNFSF18. It may not necessarily be applicable to this product.
NCBI Accession #
NP_005083.2
[Other Products]
NCBI GenBank Nucleotide #
NM_005092.3
[Other Products]
UniProt Primary Accession #
Q9UNG2
[Other Products]
UniProt Secondary Accession #
O95852; Q6ISV1; A9IQG8[Other Products]
UniProt Related Accession #
Q9UNG2[Other Products]
Molecular Weight
14.4 kDa
NCBI Official Full Name
tumor necrosis factor ligand superfamily member 18
NCBI Official Synonym Full Names
tumor necrosis factor (ligand) superfamily, member 18
NCBI Official Symbol
TNFSF18??[Similar Products]
NCBI Official Synonym Symbols
TL6; AITRL; GITRL; hGITRL
??[Similar Products]
NCBI Protein Information
tumor necrosis factor ligand superfamily member 18
UniProt Protein Name
Tumor necrosis factor ligand superfamily member 18
UniProt Synonym Protein Names
Activation-inducible TNF-related ligand; AITRL; Glucocorticoid-induced TNF-related ligand; hGITRL
Protein Family
Tumor necrosis factor ligand superfamily
UniProt Gene Name
TNFSF18??[Similar Products]
UniProt Synonym Gene Names
AITRL; GITRL; TL6; AITRL; hGITRL??[Similar Products]
UniProt Entry Name
TNF18_HUMAN
NCBI Summary for TNFSF18
The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptor TNFRSF18/AITR/GITR. It has been shown to modulate T lymphocyte survival in peripheral tissues. This cytokine is also found to be expressed in endothelial cells, and is thought to be important for interaction between T lymphocytes and endothelial cells. [provided by RefSeq, Jul 2008]
UniProt Comments for TNFSF18
TNFSF18: Cytokine that binds to TNFRSF18/AITR/GITR. Regulates T- cell responses. Can function as costimulator and lower the threshold for T-cell activation and T-cell proliferation. Important for interactions between activated T-lymphocytes and endothelial cells. Mediates activation of NF-kappa-B. Belongs to the tumor necrosis factor family.
Protein type: Membrane protein, integral; Cytokine; Apoptosis
Chromosomal Location of Human Ortholog: 1q23
Cellular Component: extracellular space; cell surface; integral to membrane; plasma membrane
Molecular Function: cytokine activity; tumor necrosis factor receptor superfamily binding; tumor necrosis factor receptor binding; receptor binding
Biological Process: cell-cell signaling; tumor necrosis factor-mediated signaling pathway; T cell proliferation during immune response; regulation of T cell proliferation; signal transduction; activation of NF-kappaB transcription factor; negative regulation of apoptosis
Research Articles on TNFSF18
1. An increase in GITRL may impair the balance of Th17/Treg, and contribute to the pathopoiesis of Hashimoto's thyroiditis.
Precautions
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Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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