Product Synonym Names
HumORF8; SPG59; UBPY
Product Gene Name
anti-USP8 antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P40818
Species Reactivity
Human, Mouse
Purity/Purification
Affinity purification
Immunogen
Recombinant protein of human USP8
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Preparation and Storage
Aliquot and store at-20 degree C. Avoid repeated freeze/thaw cycles.
Other Notes
Small volumes of anti-USP8 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-USP8 antibody
This gene encodes a protein that belongs to the ubiquitin-specific processing protease family of proteins. The encoded protein is thought to regulate the morphology of the endosome by ubiquitination of proteins on this organelle and is involved in cargo sorting and membrane trafficking at the early endosome stage. This protein is required for the cell to enter the S phase of the cell cycle and also functions as a positive regulator in the Hedgehog signaling pathway in development. Pseudogenes of this gene are present on chromosomes 2 and 6. Alternate splicing results in multiple transcript variants.
Applications Tested/Suitable for anti-USP8 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes for anti-USP8 antibody
WB: 1:500-1:2000
IHC: 1:50-1:200
IF: 1:50-1:200
NCBI/Uniprot data below describe general gene information for USP8. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001122082.1
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NCBI GenBank Nucleotide #
NM_001128610.2
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UniProt Primary Accession #
P40818
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UniProt Secondary Accession #
Q2TB31; Q7Z3U2; Q86VA0; Q8IWI7; B4DKA8[Other Products]
UniProt Related Accession #
P40818[Other Products]
NCBI Official Full Name
ubiquitin carboxyl-terminal hydrolase 8 isoform a
NCBI Official Synonym Full Names
ubiquitin specific peptidase 8
NCBI Official Symbol
USP8??[Similar Products]
NCBI Official Synonym Symbols
UBPY; PITA4; SPG59; HumORF8
??[Similar Products]
NCBI Protein Information
ubiquitin carboxyl-terminal hydrolase 8
UniProt Protein Name
Ubiquitin carboxyl-terminal hydrolase 8
UniProt Synonym Protein Names
Deubiquitinating enzyme 8; Ubiquitin isopeptidase Y; hUBPy; Ubiquitin thioesterase 8; Ubiquitin-specific-processing protease 8
UniProt Gene Name
USP8??[Similar Products]
UniProt Synonym Gene Names
KIAA0055; UBPY; hUBPy??[Similar Products]
NCBI Summary for USP8
This gene encodes a protein that belongs to the ubiquitin-specific processing protease family of proteins. The encoded protein is thought to regulate the morphology of the endosome by ubiquitination of proteins on this organelle and is involved in cargo sorting and membrane trafficking at the early endosome stage. This protein is required for the cell to enter the S phase of the cell cycle and also functions as a positive regulator in the Hedgehog signaling pathway in development. Pseudogenes of this gene are present on chromosomes 2 and 6. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]
UniProt Comments for USP8
Hydrolase that can remove conjugated ubiquitin from proteins and therefore plays an important regulatory role at the level of protein turnover by preventing degradation. Converts both 'Lys-48' an 'Lys-63'-linked ubiquitin chains. Catalytic activity is enhanced in the M phase. Involved in cell proliferation. Required to enter into S phase in response to serum stimulation. May regulate T-cell anergy mediated by RNF128 via the formation of a complex containing RNF128 and OTUB1. Probably regulates the stability of STAM2 and RASGRF1. Regulates endosomal ubiquitin dynamics, cargo sorting, membrane traffic at early endosomes, and maintenance of ESCRT-0 stability. The level of protein ubiquitination on endosomes is essential for maintaining the morphology of the organelle. Deubiquitinates EPS15 and controles tyrosine kinase stability. Removes conjugated ubiquitin from EGFR thus regulating EGFR degradation and downstream MAPK signaling. Involved in acrosome biogenesis through interaction with the spermatid ESCRT-0 complex and microtubules. Deubiquitinates BIRC6/bruce and KIF23/MKLP1.
Research Articles on USP8
1. the EGFR-USP8-trichoplein-Aurora A axis is a critical signaling cascade that restricts ciliogenesis in dividing cells, and functions to facilitate cell proliferation
Precautions
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Disclaimer
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