Full Product Name
USP8 Antibody
Product Gene Name
anti-USP8 antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Species Reactivity
Human
Predicted: Mouse, Rat
Form/Format
Purified by protein affinity column containing 1% BSA and 0.02% NaN3
Preparation and Storage
Already reconstituted in 200ul sterile H2O. The antibody is stable for at least one year when stored at-20 degree C after reconstitution. Avoid repeated freeze/defrost cycles.
Other Notes
Small volumes of anti-USP8 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-USP8 antibody
Rabbit polyclonal USP8 (1) antibody was raised against a recombinate human USP8 protein 886-1096aa (BC110590).
Product Categories/Family for anti-USP8 antibody
Autophagy antibody; Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells
Applications Tested/Suitable for anti-USP8 antibody
ELISA (EIA), Immunohistochemistry (IHC)
Application Notes for anti-USP8 antibody
ELISA: 1:20,000-1:80,000
IHC: 1:100-1:500
Testing Data of anti-USP8 antibody
NCBI/Uniprot data below describe general gene information for USP8. It may not necessarily be applicable to this product.
NCBI Accession #
BC110590
[Other Products]
UniProt Secondary Accession #
Q2TB31; Q7Z3U2; Q86VA0; Q8IWI7; B4DKA8[Other Products]
UniProt Related Accession #
P40818[Other Products]
Molecular Weight
115,080 Da
NCBI Official Full Name
Homo sapiens ubiquitin specific peptidase 8, mRNA
NCBI Official Synonym Full Names
ubiquitin specific peptidase 8
NCBI Official Symbol
USP8??[Similar Products]
NCBI Official Synonym Symbols
UBPY; PITA4; SPG59; HumORF8
??[Similar Products]
NCBI Protein Information
ubiquitin carboxyl-terminal hydrolase 8
UniProt Protein Name
Ubiquitin carboxyl-terminal hydrolase 8
UniProt Synonym Protein Names
Deubiquitinating enzyme 8; Ubiquitin isopeptidase Y; hUBPy; Ubiquitin thioesterase 8; Ubiquitin-specific-processing protease 8
UniProt Gene Name
USP8??[Similar Products]
UniProt Synonym Gene Names
KIAA0055; UBPY; hUBPy??[Similar Products]
NCBI Summary for USP8
This gene encodes a protein that belongs to the ubiquitin-specific processing protease family of proteins. The encoded protein is thought to regulate the morphology of the endosome by ubiquitination of proteins on this organelle and is involved in cargo sorting and membrane trafficking at the early endosome stage. This protein is required for the cell to enter the S phase of the cell cycle and also functions as a positive regulator in the Hedgehog signaling pathway in development. Pseudogenes of this gene are present on chromosomes 2 and 6. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]
UniProt Comments for USP8
Hydrolase that can remove conjugated ubiquitin from proteins and therefore plays an important regulatory role at the level of protein turnover by preventing degradation. Converts both 'Lys-48' an 'Lys-63'-linked ubiquitin chains. Catalytic activity is enhanced in the M phase. Involved in cell proliferation. Required to enter into S phase in response to serum stimulation. May regulate T-cell anergy mediated by RNF128 via the formation of a complex containing RNF128 and OTUB1. Probably regulates the stability of STAM2 and RASGRF1. Regulates endosomal ubiquitin dynamics, cargo sorting, membrane traffic at early endosomes, and maintenance of ESCRT-0 stability. The level of protein ubiquitination on endosomes is essential for maintaining the morphology of the organelle. Deubiquitinates EPS15 and controles tyrosine kinase stability. Removes conjugated ubiquitin from EGFR thus regulating EGFR degradation and downstream MAPK signaling. Involved in acrosome biogenesis through interaction with the spermatid ESCRT-0 complex and microtubules. Deubiquitinates BIRC6/bruce and KIF23/MKLP1.
Product References and Citations for anti-USP8 antibody
(1) Xia, R., Jia, H., Fan, J., Liu, Y. and Jia, J. USP8 promotes smoothened signaling by preventing its ubiquitination and changing its subcellular localization. PLoS Biol. 10 (1), E1001238 (2012).
Research Articles on USP8
1. the EGFR-USP8-trichoplein-Aurora A axis is a critical signaling cascade that restricts ciliogenesis in dividing cells, and functions to facilitate cell proliferation
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
