Deubiquitinating enzyme 8; hUBPy; HumORF8; KIAA0055; MGC129718; Ubiquitin carboxyl-terminal hydrolase 8; Ubiquitin-specific processing protease 8; Ubiquitin thioesterase 8; UBP; Ubiquitin Specific Protease 8

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

IgG

Chicken

Purified from egg yolk

50 ug of antibody in PBS containing 10% glycerol
Appearance: Colorless liquid

At -20 degree C
Shelf Life: 12 months

Small volumes of anti-USP8 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Polyclonal antibody to detect USP8 (a DUB enzyme) in human samples.

Background: Ubiquitinating enzymes (UBEs) catalyze protein ubiquitination, a reversible process countered by deubiquitinating enzyme (DUB) action. Five DUB subfamilies are recognized, including the USP, UCH, OTU, MJD, and JAMM enzymes. The deubiquitinating enzyme ubiquitin-specific protease 8 (USP8/UBPy) is a cysteine protease belonging to the USP/UBP subfamily. Research studies have shown that USP8 is an essential growth-regulated enzyme indispensible for cell proliferation and survival. Indeed, conditional knock-out of murine USP8 was shown to promote a dramatic loss in expression of receptor tyrosine kinases, including EGFR, ErbB3, and c-Met. In agreement with these findings, USP8 inactivation leads to enhanced ubiquitination of ligand-activated EGFR. Furthermore, phosphorylation of USP8 at Ser680 results in its binding of 14-3-3, catalytic inactivation, and reduced EGFR deubiquitination. It appears as though USP8, in conjunction with components of the ESCRT-0 complex, plays an integral role in the early endosomal sorting machinery that functions to protect EGFR from lysosomal degradation.

Epigenetics; Eraser Enzymes; Ubiquitin-Specific Proteases; AntibodiesAntibodies & Supporting Tools; Primary Antibodies (A-Z)

Western Blot (WB)

Western blot: Robust detection of 100 ng of recombinant protein was possible when antibody was used at a final concentration of 5 mug/mL

115,080 Da

ubiquitin specific peptidase 8

ubiquitin carboxyl-terminal hydrolase 8

Ubiquitin carboxyl-terminal hydrolase 8

KIAA0055; UBPY; hUBPy??[Similar Products]

UBP8_HUMAN

This gene encodes a protein that belongs to the ubiquitin-specific processing protease family of proteins. The encoded protein is thought to regulate the morphology of the endosome by ubiquitination of proteins on this organelle and is involved in cargo sorting and membrane trafficking at the early endosome stage. This protein is required for the cell to enter the S phase of the cell cycle and also functions as a positive regulator in the Hedgehog signaling pathway in development. Pseudogenes of this gene are present on chromosomes 2 and 6. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]

USP8: Hydrolase that can remove conjugated ubiquitin from proteins and therefore plays an important regulatory role at the level of protein turnover by preventing degradation. Converts both 'Lys-48' an 'Lys-63'-linked ubiquitin chains. Catalytic activity is enhanced in the M phase. Involved in cell proliferation. Required to enter into S phase in response to serum stimulation. May regulate T-cell anergy mediated by RNF128 via the formation of a complex containing RNF128 and OTUB1. Probably regulates the stability of STAM2 and RASGRF1. Regulates endosomal ubiquitin dynamics, cargo sorting, membrane traffic at early endosomes, and maintenance of ESCRT-0 stability. The level of protein ubiquitination on endosomes is essential for maintaining the morphology of the organelle. Deubiquitinates EPS15 and controles tyrosine kinase stability. Removes conjugated ubiquitin from EGFR thus regulating EGFR degradation and downstream MAPK signaling. Involved in acrosome biogenesis through interaction with the spermatid ESCRT-0 complex and microtubules. Deubiquitinates BIRC6/bruce and KIF23/MKLP1. Forms a ternary complex with RNF128 and OTUB1. Interacts (via C-terminal UCH catalytic domain) with OTUB1 isoform 1. Interacts with STAM2 (via SH3 domain). Interacts with DNAJB3, EGFR, EPS15, RASGRF1, RNF41, YWHAE, YWHAG and YWHAZ. Interacts with NBR1, RASGRF1, RNF41 and IST1. Associates with the ESCRT-0 complex and with microtubules. Interacts with BIRC6/bruce and KIF23/MKLP1. Upon growth stimulation in starved human fibroblasts. Decreases in response to growth arrest induced by cell-cell contact. Belongs to the peptidase C19 family.

Protein type: Ubiquitin conjugating system; Ubiquitin-specific protease; EC 3.4.19.12; Protease

Chromosomal Location of Human Ortholog: 15q21.2

Cellular Component: nucleoplasm; extrinsic to plasma membrane; Golgi apparatus; cytoplasm; early endosome; acrosome; midbody; cytosol

Molecular Function: protein binding; cysteine-type endopeptidase activity; ubiquitin-specific protease activity; SH3 domain binding

Biological Process: cell proliferation; proteasomal ubiquitin-dependent protein catabolic process; protein deubiquitination; cytokinesis after mitosis; endosome organization and biogenesis; Ras protein signal transduction

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