Methyl-CpG-binding protein 2; MeCp-2 protein; MeCp2; MECP2

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit Ig

Rabbit

This MeCP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 58-87 amino acids from the N-terminal region of human MeCP2.

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Vial Concentration: 0.5 (lot specific)

Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Small volumes of anti-MECP2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

DNA methylation is the major modification of eukaryotic
genomes and plays an essential role in mammalian development. Human
proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of
nuclear proteins related by the presence in each of a methyl-CpG
binding domain (MBD). Each of these proteins, with the exception of
MBD3, is capable of binding specifically to methylated DNA. MECP2,
MBD1 and MBD2 can also repress transcription from methylated gene
promoters. In contrast to other MBD family members, MECP2 is
X-linked and subject to X inactivation. MECP2 is dispensible in
stem cells, but is essential for embryonic development. MECP2 gene
mutations are the cause of most cases of Rett syndrome, a
progressive neurologic developmental disorder and one of the most
common causes of mental retardation in females. [provided by
RefSeq].

Neuroscience

Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)

WB~~1:1000

MeCP2 Antibody (pS80) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the MeCP2 antibody detected the MeCP2 protein (arrow).

MeCP2 Antibody (N-term S80) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MeCP2 Antibody (N-term S80) for immunohistochemistry. Clinical relevance has not been evaluated.

MeCP2 Antibody (N-term S80) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

52441

methyl CpG binding protein 2

methyl-CpG-binding protein 2

Methyl-CpG-binding protein 2

MeCp-2 protein; MeCp2??[Similar Products]

MECP2_HUMAN

DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. In contrast to other MBD family members, MECP2 is X-linked and subject to X inactivation. MECP2 is dispensible in stem cells, but is essential for embryonic development. MECP2 gene mutations are the cause of most cases of Rett syndrome, a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females. [provided by RefSeq, Jul 2009]

MECP2: Chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair. It is not influenced by sequences flanking the methyl-CpGs. Mediates transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A. Interacts with FNBP3. Interacts with CDKL5. Present in all ***** somatic tissues tested. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: Xq28

Cellular Component: nucleoplasm; heterochromatin; extracellular space; cytosol; nucleus

Molecular Function: mRNA binding; protein domain specific binding; protein binding; siRNA binding; DNA binding; methyl-CpG binding; double-stranded methylated DNA binding; protein N-terminus binding; chromatin binding; transcription factor activity; transcription factor binding; transcription corepressor activity

Biological Process: catecholamine secretion; genetic imprinting; behavioral fear response; startle response; ***** locomotory behavior; positive regulation of transcription, DNA-dependent; chromatin silencing; pathogenesis; neurological control of breathing; negative regulation of transcription from RNA polymerase II promoter; proprioception; sensory perception of pain; negative regulation of histone methylation; neuron maturation; negative regulation of histone acetylation; phosphatidylcholine metabolic process; post-embryonic development; protein localization; synaptogenesis; positive regulation of cell proliferation; dendrite development; cerebellum development; visual learning; negative regulation of neuron apoptosis; histone acetylation; mitochondrial electron transport, ubiquinol to cytochrome c; transcription, DNA-dependent; ventricular system development; glutamine metabolic process; social behavior; respiratory gaseous exchange; cardiolipin metabolic process; histone methylation; glucocorticoid metabolic process; positive regulation of synaptogenesis; regulation of systemic arterial blood pressure by neurological process; long-term memory; inositol metabolic process; response to hypoxia; regulation of excitatory postsynaptic membrane potential; negative regulation of transcription, DNA-dependent

Disease: Rett Syndrome; Mental Retardation, X-linked, Syndromic 13; Autism, Susceptibility To, X-linked 3; Lubs X-linked Mental Retardation Syndrome; Encephalopathy, Neonatal Severe, Due To Mecp2 Mutations; Angelman Syndrome

Shapiro, J.R., et al. Pediatr. Res. 68(5):446-451(2010)
Pintaudi, M., et al. Epilepsy Behav (2010) In press :
Jain, D., et al. Pediatr. Neurol. 43(1):35-40(2010)
Harvey, C.G., et al. Am. J. Med. Genet. B Neuropsychiatr. Genet. 144B (3), 355-360 (2007) :
Francke, U. Nat Clin Pract Neurol 2(4):212-221(2006)

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