For Research Use Only. Not for use in diagnostic procedures.

This kit recognizes natural and recombinantHumanMeCP2. No significant cross-reactivity or interference between HumanMeCP2 and analogues was observed.

Store at 4 degree C.

Manufactured in an ISO 9001:2015 Certified Laboratory.

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of MeCP2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS2515729 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the MeCP2, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing MeCP2. The ELISA analytical biochemical technique of the MBS2515729 kit is based on MeCP2 antibody-MeCP2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MeCP2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MeCP2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Intended Uses: This ELISA kit applies to the invitro quantitative determination of HumanMeCP2concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to MeCP2. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for MeCP2and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain MeCP2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of MeCP2.You can calculate the concentration of MeCP2in the samples by comparing the OD of the samples to the standard curve.

53,323 Da

methyl CpG binding protein 2

methyl-CpG-binding protein 2; meCp-2 protein

Methyl-CpG-binding protein 2

MeCp-2 protein; MeCp2??[Similar Products]

MECP2_HUMAN

DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. In contrast to other MBD family members, MECP2 is X-linked and subject to X inactivation. MECP2 is dispensible in stem cells, but is essential for embryonic development. MECP2 gene mutations are the cause of most cases of Rett syndrome, a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females. [provided by RefSeq, Jul 2009]

MECP2: Chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair. It is not influenced by sequences flanking the methyl-CpGs. Mediates transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A. Interacts with FNBP3. Interacts with CDKL5. Present in all ***** somatic tissues tested. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: Xq28

Cellular Component: nucleoplasm; heterochromatin; extracellular space; nucleus; cytosol

Molecular Function: mRNA binding; protein domain specific binding; protein binding; siRNA binding; methyl-CpG binding; DNA binding; double-stranded methylated DNA binding; protein N-terminus binding; chromatin binding; transcription corepressor activity; transcription factor binding; transcription factor activity

Biological Process: catecholamine secretion; startle response; genetic imprinting; behavioral fear response; ***** locomotory behavior; positive regulation of transcription, DNA-dependent; chromatin silencing; pathogenesis; neurological control of breathing; negative regulation of transcription from RNA polymerase II promoter; proprioception; sensory perception of pain; negative regulation of histone methylation; neuron maturation; post-embryonic development; negative regulation of histone acetylation; phosphatidylcholine metabolic process; protein localization; synaptogenesis; positive regulation of cell proliferation; dendrite development; visual learning; cerebellum development; negative regulation of neuron apoptosis; histone acetylation; mitochondrial electron transport, ubiquinol to cytochrome c; transcription, DNA-dependent; ventricular system development; glutamine metabolic process; respiratory gaseous exchange; social behavior; cardiolipin metabolic process; histone methylation; positive regulation of synaptogenesis; glucocorticoid metabolic process; regulation of systemic arterial blood pressure by neurological process; long-term memory; inositol metabolic process; response to hypoxia; regulation of excitatory postsynaptic membrane potential; negative regulation of transcription, DNA-dependent

Disease: Rett Syndrome; Mental Retardation, X-linked, Syndromic 13; Autism, Susceptibility To, X-linked 3; Lubs X-linked Mental Retardation Syndrome; Encephalopathy, Neonatal Severe, Due To Mecp2 Mutations; Angelman Syndrome

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