Product Name
MECP2, siRNA
Full Product Name
MECP2 siRNA (Mouse)
Product Synonym Names
Methyl-CpG-binding protein 2; MeCp-2 protein; MeCp2
Product Gene Name
MECP2 sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q9Z2D6
Specificity
MECP2 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse MECP2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of MECP2 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
MECP2 sirna
siRNA to inhibit MECP2 expression using RNA interference
Applications Tested/Suitable for MECP2 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for MECP2. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001075448.1
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NCBI GenBank Nucleotide #
NM_001081979.2
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UniProt Primary Accession #
Q9Z2D6
[Other Products]
UniProt Secondary Accession #
Q3TYG1; B1AUZ2; B1AUZ3[Other Products]
UniProt Related Accession #
Q9Z2D6[Other Products]
Molecular Weight
53,576 Da
NCBI Official Full Name
methyl-CpG-binding protein 2 isoform 1
NCBI Official Synonym Full Names
methyl CpG binding protein 2
NCBI Official Symbol
Mecp2??[Similar Products]
NCBI Official Synonym Symbols
Mbd5; WBP10; 1500041B07Rik; D630021H01Rik
??[Similar Products]
NCBI Protein Information
methyl-CpG-binding protein 2
UniProt Protein Name
Methyl-CpG-binding protein 2
Protein Family
Methyl-CpG-binding protein
UniProt Gene Name
Mecp2??[Similar Products]
UniProt Synonym Gene Names
MeCp-2 protein; MeCp2??[Similar Products]
UniProt Entry Name
MECP2_MOUSE
UniProt Comments for MECP2
MECP2: Chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair. It is not influenced by sequences flanking the methyl-CpGs. Mediates transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A. Interacts with FNBP3. Interacts with CDKL5. Present in all ***** somatic tissues tested. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Transcription, coactivator/corepressor
Cellular Component: nucleoplasm; heterochromatin; extracellular space; protein complex; cell; cytoplasm; nuclear chromatin; cytosol; nucleus
Molecular Function: protein domain specific binding; siRNA binding; methyl-CpG binding; unmethylated CpG binding; chromatin DNA binding; histone deacetylase binding; protein N-terminus binding; transcription factor binding; mRNA binding; protein binding; enzyme binding; DNA binding; four-way junction DNA binding; double-stranded methylated DNA binding; chromatin binding; transcription factor activity
Biological Process: catecholamine secretion; genetic imprinting; ***** locomotory behavior; positive regulation of transcription, DNA-dependent; regulation of synaptic plasticity; neurological control of breathing; sensory perception of pain; neuron maturation; post-embryonic development; neuron differentiation; neuromuscular process controlling posture; protein localization; cerebellum development; histone acetylation; negative regulation of neuron apoptosis; neurite development; mitochondrial electron transport, ubiquinol to cytochrome c; transcription, DNA-dependent; ventricular system development; social behavior; respiratory gaseous exchange; positive regulation of synaptogenesis; regulation of gene expression; negative regulation of transcription, DNA-dependent; multicellular organismal response to stress; behavioral fear response; startle response; chromatin silencing; pathogenesis; proprioception; negative regulation of transcription from RNA polymerase II promoter; regulation of gene expression, epigenetic; negative regulation of histone methylation; negative regulation of histone acetylation; phosphatidylcholine metabolic process; synaptic transmission; biogenic amine metabolic process; synaptogenesis; regulation of transcription, DNA-dependent; positive regulation of cell proliferation; dendrite development; visual learning; neuromuscular process; glutamine metabolic process; learning; cardiolipin metabolic process; memory; histone methylation; glucocorticoid metabolic process; embryonic development; regulation of systemic arterial blood pressure by neurological process; long-term memory; inositol metabolic process; negative regulation of astrocyte differentiation; response to hypoxia; brain development; regulation of excitatory postsynaptic membrane potential
Research Articles on MECP2
1. Increased levels of TNFalpha, IL-6, and IL-3 mRNAs were observed in splenocytes from MeCP2-null mice.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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