Product Name
BARX1, siRNA
Full Product Name
BARX1 siRNA (Human)
Product Synonym Names
Homeobox protein BarH-like 1
Product Gene Name
BARX1 sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q9HBU1
Specificity
BARX1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human BARX1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of BARX1 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
BARX1 sirna
siRNA to inhibit BARX1 expression using RNA interference
Applications Tested/Suitable for BARX1 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for BARX1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_067545.3
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NCBI GenBank Nucleotide #
NM_021570.3
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UniProt Primary Accession #
Q9HBU1
[Other Products]
UniProt Secondary Accession #
Q6P2R4; Q96GH8[Other Products]
UniProt Related Accession #
Q9HBU1[Other Products]
Molecular Weight
11,481 Da
NCBI Official Full Name
homeobox protein BarH-like 1
NCBI Official Synonym Full Names
BARX homeobox 1
NCBI Official Symbol
BARX1??[Similar Products]
NCBI Protein Information
homeobox protein BarH-like 1
UniProt Protein Name
Homeobox protein BarH-like 1
Protein Family
Homeobox protein
UniProt Gene Name
BARX1??[Similar Products]
UniProt Entry Name
BARX1_HUMAN
NCBI Summary for BARX1
This gene encodes a member of the Bar subclass of homeobox transcription factors. Studies of the mouse and chick homolog suggest the encoded protein may play a role in developing teeth and craniofacial mesenchyme of neural crest origin. The protein may also be associated with differentiation of stomach epithelia. [provided by RefSeq, Jul 2008]
UniProt Comments for BARX1
BARX1: Transcription factor, which is involved in craniofacial development, in odontogenesis and in stomach organogenesis. May have a role in the differentiation of molars from incisors. Plays a role in suppressing endodermal Wnt activity. Binds to a regulatory module of the NCAM promoter. Belongs to the BAR homeobox family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: DNA-binding; Transcription factor
Chromosomal Location of Human Ortholog: 9q12
Cellular Component: nucleus
Molecular Function: sequence-specific DNA binding; transcription factor activity
Biological Process: spleen development; anterior/posterior pattern formation; negative regulation of Wnt receptor signaling pathway; epithelial cell differentiation; regulation of transcription, DNA-dependent; cell-cell signaling; transcription, DNA-dependent
Research Articles on BARX1
1. Regional expression of barx1 was observed in epithelium before mixed dentition, while during mixed dentition gene appeared in hyaline cartilage. Expression of barx1 appears in cleft lip palate affected structures mainly in mixed dentition.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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