Full Product Name
RP9 siRNA (Human)
Product Synonym Names
Retinitis pigmentosa 9 protein; Pim-1-associated protein; PAP-1
Product Gene Name
RP9 sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q8TA86
Specificity
RP9 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human RP9 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of RP9 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
RP9 sirna
siRNA to inhibit RP9 expression using RNA interference
Applications Tested/Suitable for RP9 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for RP9. It may not necessarily be applicable to this product.
NCBI Accession #
NP_976033.1
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NCBI GenBank Nucleotide #
NM_203288.1
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UniProt Primary Accession #
Q8TA86
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UniProt Related Accession #
Q8TA86[Other Products]
Molecular Weight
26,107 Da
NCBI Official Full Name
retinitis pigmentosa 9 protein
NCBI Official Synonym Full Names
retinitis pigmentosa 9 (autosomal dominant)
NCBI Official Symbol
RP9??[Similar Products]
NCBI Official Synonym Symbols
PAP1; PAP-1
??[Similar Products]
NCBI Protein Information
retinitis pigmentosa 9 protein
UniProt Protein Name
Retinitis pigmentosa 9 protein
UniProt Synonym Protein Names
Pim-1-associated protein; PAP-1
Protein Family
Retinitis pigmentosa 9 protein
UniProt Gene Name
RP9??[Similar Products]
UniProt Synonym Gene Names
PAP-1??[Similar Products]
UniProt Entry Name
RP9_HUMAN
NCBI Summary for RP9
The protein encoded by this gene can be bound and phosphorylated by the protooncogene PIM1 product, a serine/threonine protein kinase . This protein localizes in nuclear speckles containing the splicing factors, and has a role in pre-mRNA splicing. CBF1-interacting protein (CIR), a corepressor of CBF1, can also bind to this protein and effects alternative splicing. Mutations in this gene result in autosomal dominant retinitis pigmentosa-9. This gene has a pseudogene (GeneID: 441212), which is located in tandem array approximately 166 kb distal to this gene. [provided by RefSeq, Sep 2009]
UniProt Comments for RP9
RP9: Is thought to be a target protein for the PIM1 kinase. May play some roles in B-cell proliferation in association with PIM1. Defects in RP9 are the cause of retinitis pigmentosa type 9 (RP9). RP leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. RP9 inheritance is autosomal dominant.
Protein type: RNA processing
Chromosomal Location of Human Ortholog: 7p14.3
Cellular Component: nucleoplasm; cytoplasm; signal recognition particle receptor complex; nucleus
Molecular Function: protein binding; zinc ion binding
Biological Process: RNA splicing; cognition
Disease: Retinitis Pigmentosa 9
Research Articles on RP9
1. Observational study of genetic testing. (HuGE Navigator)
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
