For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

IgG

Rabbit

The antibody is a rabbit polyclonal antibody raised against MyD88. It has been selected for its ability to recognize MyD88 in immunohistochemical staining andwestern blotting.

Affinity Chromatography

Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.

200ug/ml (lot specific)

Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.

Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customera??s specifications, please inquire.

Small volumes of anti-MyD88 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)

Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200

Western Blot: Sample: Recombinant protein.

DAB staining on fromalin fixed paraffin- embedded kidney tissue)

DAB staining on IHC-P; Samples: Human Stomach Tissue)

28,683 Da

myeloid differentiation primary response gene 88

myeloid differentiation primary response protein MyD88

Myeloid differentiation primary response protein MyD88

MYD88: Adapter protein involved in the Toll-like receptor and IL-1 receptor signaling pathway in the innate immune response. Acts via IRAK1, IRAK2, IRF7 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Increases IL-8 transcription. Involved in IL-18-mediated signaling pathway. Activates IRF1 resulting in its rapid migration into the nucleus to mediate an efficient induction of IFN-beta, NOS2/INOS, and IL12A genes. Homodimer. Also forms heterodimers with TIRAP. Binds to TLR2, TLR4, IRAK1, IRAK2 and IRAK4 via their respective TIR domains. Interacts with IL18R1. Interacts with BMX, IL1RL1 and IRF7. Interacts with LRRFIP1 and LRRFIP2; this interaction positively regulates Toll-like receptor (TLR) signaling in response to agonist. Interacts with FLII. LRRFIP1 and LRRFIP2 compete with FLII for MYD88-binding. Interacts with IRF1. Ubiquitous. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 9 F3|9 71.33 cM

Cellular Component: cytoplasm; cytosol; nucleus; plasma membrane; postsynaptic density; protein complex

Molecular Function: identical protein binding; interleukin-1 receptor binding; protein binding; protein self-association; receptor binding; Toll binding

Biological Process: apoptosis; cell surface receptor signaling pathway; cytokine-mediated signaling pathway; defense response to Gram-positive bacterium; immune response; immune system process; immunoglobulin mediated immune response; induced systemic resistance; inflammatory response; innate immune response; JNK cascade; lipopolysaccharide-mediated signaling pathway; MyD88-dependent toll-like receptor signaling pathway; positive regulation of chemokine biosynthetic process; positive regulation of I-kappaB kinase/NF-kappaB signaling; positive regulation of interferon type I production; positive regulation of interleukin-17 production; positive regulation of interleukin-23 production; positive regulation of interleukin-6 production; positive regulation of JNK cascade; positive regulation of lymphocyte proliferation; positive regulation of NF-kappaB transcription factor activity; positive regulation of smooth muscle cell proliferation; positive regulation of tumor necrosis factor production; regulation of cell proliferation; regulation of gene expression; regulation of inflammatory response; regulation of interleukin-6 production; regulation of tumor necrosis factor production; response to lipopolysaccharide; response to molecule of fungal origin; response to peptidoglycan; response to virus; signal transduction; Toll signaling pathway; transmembrane receptor protein serine/threonine kinase signaling pathway; type I interferon biosynthetic process

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While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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