Myeloid differentiation primary response protein

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit

Recognizes the death domain of MyD88.

Liquid. Neat serum.

Long term: 4 degree C
Stable for at least 6 months after receipt when stored at +4 degree C undiluted. Avoid freeze/thaw cycles.

Small volumes of anti-MyD88 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Cell Signaling

Western Blot (WB)

Western Blot (1:5,000-1:8,000)
Suggested dilutions/conditions may not be available for all applications.

Figure: Detection of endogenous MyD88 in mouse EL-4 and human HEK 293T cells.Method: Cell extracts from EL-4 (1) or 293T cells (2) were resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and incubated with the antiserum to MyD88 at a dilution of 1:8000. Proteins were visualized using a peroxidase-conjugated antibody to rabbit IgG and a chemiluminescence detection system.

34,097 Da

myeloid differentiation primary response 88

myeloid differentiation primary response protein MyD88

Myeloid differentiation primary response protein MyD88

MYD88_HUMAN

This gene encodes a cytosolic adapter protein that plays a central role in the innate and adaptive immune response. This protein functions as an essential signal transducer in the interleukin-1 and Toll-like receptor signaling pathways. These pathways regulate that activation of numerous proinflammatory genes. The encoded protein consists of an N-terminal death domain and a C-terminal Toll-interleukin1 receptor domain. Patients with defects in this gene have an increased susceptibility to pyogenic bacterial infections. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Feb 2010]

MYD88: Adapter protein involved in the Toll-like receptor and IL-1 receptor signaling pathway in the innate immune response. Acts via IRAK1, IRAK2, IRF7 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Increases IL-8 transcription. Involved in IL-18-mediated signaling pathway. Activates IRF1 resulting in its rapid migration into the nucleus to mediate an efficient induction of IFN-beta, NOS2/INOS, and IL12A genes. Homodimer. Also forms heterodimers with TIRAP. Binds to TLR2, TLR4, IRAK1, IRAK2 and IRAK4 via their respective TIR domains. Interacts with IL18R1. Interacts with BMX, IL1RL1 and IRF7. Interacts with LRRFIP1 and LRRFIP2; this interaction positively regulates Toll-like receptor (TLR) signaling in response to agonist. Interacts with FLII. LRRFIP1 and LRRFIP2 compete with FLII for MYD88-binding. Interacts with IRF1. Ubiquitous. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 3p22

Cellular Component: cytosol; endosome membrane; plasma membrane

Molecular Function: death receptor binding; identical protein binding; protein binding

Biological Process: cell surface receptor linked signal transduction; defense response to Gram-positive bacterium; inflammatory response; innate immune response; MyD88-dependent toll-like receptor signaling pathway; negative regulation of apoptosis; nerve growth factor receptor signaling pathway; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of interferon type I production; positive regulation of interleukin-17 production; positive regulation of interleukin-23 production; positive regulation of interleukin-6 production; regulation of inflammatory response; signal transduction; toll-like receptor 10 signaling pathway; toll-like receptor 2 signaling pathway; toll-like receptor 4 signaling pathway; toll-like receptor 5 signaling pathway; toll-like receptor 9 signaling pathway; toll-like receptor signaling pathway

Disease: Macroglobulinemia, Waldenstrom, Susceptibility To, 1; Myd88 Deficiency

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