Product Name
LMBRD1, siRNA
Full Product Name
LMBRD1 siRNA (Human)
Product Synonym Names
C6orf209; NESI; Probable lysosomal cobalamin transporter; HDAg-L-interacting protein NESI; LMBR1 domain-containing protein 1; Nuclear export signal-interacting protein
Product Gene Name
LMBRD1 sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q9NUN5
Specificity
LMBRD1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human LMBRD1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of LMBRD1 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
LMBRD1 sirna
siRNA to inhibit LMBRD1 expression using RNA interference
Applications Tested/Suitable for LMBRD1 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for LMBRD1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_060838.3
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NCBI GenBank Nucleotide #
NM_018368.3
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UniProt Primary Accession #
Q9NUN5
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UniProt Secondary Accession #
Q5VUN6; Q86Y70; Q96FW4; Q9BY56; Q9NZD6; A8K204; E1P531[Other Products]
UniProt Related Accession #
Q9NUN5[Other Products]
Molecular Weight
21,394 Da
NCBI Official Full Name
probable lysosomal cobalamin transporter
NCBI Official Synonym Full Names
LMBR1 domain containing 1
NCBI Official Symbol
LMBRD1??[Similar Products]
NCBI Official Synonym Symbols
NESI; LMBD1; MAHCF; C6orf209
??[Similar Products]
NCBI Protein Information
probable lysosomal cobalamin transporter
UniProt Protein Name
Probable lysosomal cobalamin transporter
UniProt Synonym Protein Names
HDAg-L-interacting protein NESI; LMBR1 domain-containing protein 1; Nuclear export signal-interacting protein
UniProt Gene Name
LMBRD1??[Similar Products]
UniProt Synonym Gene Names
C6orf209; NESI??[Similar Products]
UniProt Entry Name
LMBD1_HUMAN
NCBI Summary for LMBRD1
This gene encodes a lysosomal membrane protein that may be involved in the transport and metabolism of cobalamin. This protein also interacts with the large form of the hepatitis delta antigen and may be required for the nucleocytoplasmic shuttling of the hepatitis delta virus. Mutations in this gene are associated with the vitamin B12 metabolism disorder termed, homocystinuria-megaloblastic anemia complementation type F.[provided by RefSeq, Oct 2009]
UniProt Comments for LMBRD1
LMBRD1: Probable lysosomal cobalamin transporter. Required to export cobalamin from lysosomes allowing its conversion to cofactors. Isoform 3 may play a role in the assembly of hepatitis delta virus (HDV). Defects in LMBRD1 are the cause of methylmalonic aciduria and homocystinuria type cblF (MMAHCF). A disorder of cobalamin metabolism characterized by decreased levels of the coenzymes adenosylcobalamin (AdoCbl) and methylcobalamin (MeCbl). It is due to accumulation of free cobalamin in lysosomes, thus hindering its conversion to cofactors. Clinical features include developmental delay, stomatitis, glossitis, seizures and methylmalonic aciduria responsive to vitamin B12. Belongs to the LIMR family. LMBRD1 subfamily. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, multi-pass; Membrane protein, integral
Chromosomal Location of Human Ortholog: 6q13
Cellular Component: membrane; lysosomal membrane; plasma membrane; integral to membrane; clathrin-coated endocytic vesicle
Molecular Function: cobalamin binding; insulin receptor binding
Biological Process: negative regulation of glucose import; negative regulation of protein kinase B signaling cascade; vitamin metabolic process; viral reproduction; cobalamin metabolic process; negative regulation of insulin receptor signaling pathway; water-soluble vitamin metabolic process
Disease: Methylmalonic Aciduria And Homocystinuria, Cblf Type
Research Articles on LMBRD1
1. LMBD1 plays an imperative role in mediating and regulating the endocytosis of the IR.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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