Mouse Monoclonal [clone 1B4] (IgG2a) to Human MYD88; Human MYD88; MYD88D

For Research Use Only. Not for use in diagnostic procedures.

Monoclonal

IgG2a

1B4

Mouse

Human MYD88

Protein A/G purified

PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide

1 mg/ml (lot specific)

Store at -20 degree C. Minimize freezing and thawing.

Small volumes of anti-MYD88 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MYD88 Antibody, MYD88D Antibody Description: The pro-inflammatory cytokine IL-1 induced cellular response requires IL-1 receptor complex including IL-1RI and IL-1RAcP. Recently, MyD88 was identified as an adapter molecule in the IL-1 signaling pathway. MyD88 associates with and recruits IRAK to the IL-1 receptor complex in response to IL-1 treatment and dominant negative form of MyD88 attenuates IL-1R-mediated NF-kB activation.

Immunohistochemistry (IHC) Paraffin, Immunofluorescence (IF), Western Blot (WB), Flow Cytometry (FC/FACS)

Flow Cytometry (1:100)
IF (1:100)
IHC-P (10 ug/ml)
WB (1:2000)

Anti-MYD88 antibody IHC staining of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

Anti-MYD88 antibody IHC staining of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Anti-MYD88 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MYD88.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MYD88 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MYD88.

Flow cytometry of HeLa cells, using anti-MYD88 antibody (Red), compared to a nonspecific negative control antibody (Blue).

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-MYD88 antibody, and then analyzed by flow cytometry.

Flow cytometry of Jurkat cells, using anti-MYD88 antibody (Red), compared to a nonspecific negative control antibody (Blue).

34,097 Da

myeloid differentiation primary response 88

myeloid differentiation primary response protein MyD88

Myeloid differentiation primary response protein MyD88

MYD88_HUMAN

This gene encodes a cytosolic adapter protein that plays a central role in the innate and adaptive immune response. This protein functions as an essential signal transducer in the interleukin-1 and Toll-like receptor signaling pathways. These pathways regulate that activation of numerous proinflammatory genes. The encoded protein consists of an N-terminal death domain and a C-terminal Toll-interleukin1 receptor domain. Patients with defects in this gene have an increased susceptibility to pyogenic bacterial infections. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Feb 2010]

MYD88: Adapter protein involved in the Toll-like receptor and IL-1 receptor signaling pathway in the innate immune response. Acts via IRAK1, IRAK2, IRF7 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Increases IL-8 transcription. Involved in IL-18-mediated signaling pathway. Activates IRF1 resulting in its rapid migration into the nucleus to mediate an efficient induction of IFN-beta, NOS2/INOS, and IL12A genes. Homodimer. Also forms heterodimers with TIRAP. Binds to TLR2, TLR4, IRAK1, IRAK2 and IRAK4 via their respective TIR domains. Interacts with IL18R1. Interacts with BMX, IL1RL1 and IRF7. Interacts with LRRFIP1 and LRRFIP2; this interaction positively regulates Toll-like receptor (TLR) signaling in response to agonist. Interacts with FLII. LRRFIP1 and LRRFIP2 compete with FLII for MYD88-binding. Interacts with IRF1. Ubiquitous. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 3p22

Cellular Component: cytosol; endosome membrane; plasma membrane

Molecular Function: death receptor binding; identical protein binding; protein binding

Biological Process: cell surface receptor linked signal transduction; defense response to Gram-positive bacterium; inflammatory response; innate immune response; MyD88-dependent toll-like receptor signaling pathway; negative regulation of apoptosis; nerve growth factor receptor signaling pathway; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of interferon type I production; positive regulation of interleukin-17 production; positive regulation of interleukin-23 production; positive regulation of interleukin-6 production; regulation of inflammatory response; signal transduction; toll-like receptor 10 signaling pathway; toll-like receptor 2 signaling pathway; toll-like receptor 4 signaling pathway; toll-like receptor 5 signaling pathway; toll-like receptor 9 signaling pathway; toll-like receptor signaling pathway

Disease: Macroglobulinemia, Waldenstrom, Susceptibility To, 1; Myd88 Deficiency

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