Full Product Name
BLyS antibody
Product Synonym Names
B lymphocyte stimulator; B-cell activating factor; BAFF; CD257; TALL1; THANK; ZTNF4; TNFSF13B; TNFSF20; BlyS; B lymphocyte stimulator; B lymphocyte stimulator
Product Gene Name
anti-BLyS antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q9Y275
Species Reactivity
Human, Rat
Purity/Purification
By protein-G affinity chromatography
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Concentration
1mg/ml (lot specific)
Immunogen
Recombinant human BLyS (134-285aa) purified from E. coli
Clone Note
Anti-human BLyS mAb, clone H4-C7, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human BLyS protein.
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Other Notes
Small volumes of anti-BLyS antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-BLyS antibody
B lymphocyte stimulator (BLyS),a member of the tumor necrosis factor (TNF) superfamily of cytokines, induces B-cell proliferation and immunoglobulin secretion and is a key regulator of peripheral B-cell populations in vivo.. BLyS is made by immune-cells called monocytes and macrophages. When monocytes and macrophages are activated, BLyS is released and binds to a receptor found only on B cells.
Product Categories/Family for anti-BLyS antibody
Hormones & Cytokines
Applications Tested/Suitable for anti-BLyS antibody
ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes for anti-BLyS antibody
WB: 1:500 ~ 1,000.
Recommended starting dilution is 1:500.
Western Blot (WB) of anti-BLyS antibody
Tissue lysates of Rat spleen (60ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human BLyS (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Immunofluorescence (IF) of anti-BLyS antibody
ICC/IF analysis of BLyS in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human BLyS antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green)
NCBI/Uniprot data below describe general gene information for BLyS. It may not necessarily be applicable to this product.
NCBI Accession #
NP_006564
[Other Products]
NCBI GenBank Nucleotide #
NM_006573.4
[Other Products]
UniProt Primary Accession #
Q9Y275
[Other Products]
UniProt Secondary Accession #
Q6FHD6; Q7Z5J2; E0ADT7[Other Products]
UniProt Related Accession #
Q9Y275[Other Products]
Molecular Weight
17,578 Da
NCBI Official Full Name
tumor necrosis factor ligand superfamily member 13B isoform 1
NCBI Official Synonym Full Names
TNF superfamily member 13b
NCBI Official Symbol
TNFSF13B??[Similar Products]
NCBI Official Synonym Symbols
DTL; BAFF; BLYS; CD257; TALL1; THANK; ZTNF4; TALL-1; TNLG7A; TNFSF20
??[Similar Products]
NCBI Protein Information
tumor necrosis factor ligand superfamily member 13B
UniProt Protein Name
Tumor necrosis factor ligand superfamily member 13B
UniProt Synonym Protein Names
B lymphocyte stimulator; BLyS
UniProt Gene Name
TNFSF13B??[Similar Products]
UniProt Synonym Gene Names
BAFF; BLYS; TALL1; TNFSF20; ZTNF4; BLyS; TALL-1??[Similar Products]
NCBI Summary for BLyS
The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFFR. This cytokine is expressed in B cell lineage cells, and acts as a potent B cell activator. It has been also shown to play an important role in the proliferation and differentiation of B cells. Alternatively spliced transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Mar 2011]
UniProt Comments for BLyS
Cytokine that binds to TNFRSF13B/TACI and TNFRSF17/BCMA. TNFSF13/APRIL binds to the same 2 receptors. Together, they form a 2 ligands -2 receptors pathway involved in the stimulation of B- and T-cell function and the regulation of humoral immunity. A third B-cell specific BAFF-receptor (BAFFR/BR3) promotes the survival of mature B-cells and the B-cell response.
Product References and Citations for anti-BLyS antibody
Allan SM., et al. (2005), Nat. Rev. Immunol. 5:629-40.; Michaud DS., et al. (2006), Cancer Res. 66 (8):4525-30
Research Articles on BLyS
1. soluble BCMA sequesters circulating BAFF, thereby preventing it from performing its signaling to stimulate normal B-cell and plasma cell development, resulting in reduced polyclonal antibody levels in multiple myeloma patients.
Precautions
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Disclaimer
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