Full Product Name
OTOF siRNA (Human)
Product Synonym Names
FER1L2; Otoferlin; Fer-1-like protein 2
Product Gene Name
OTOF sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q9HC10
Specificity
OTOF siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human OTOF gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of OTOF sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
OTOF sirna
siRNA to inhibit OTOF expression using RNA interference
Applications Tested/Suitable for OTOF sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for OTOF. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001274418.1
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NCBI GenBank Nucleotide #
NM_001287489.1
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UniProt Primary Accession #
Q9HC10
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UniProt Secondary Accession #
Q53R90; Q9HC08; Q9HC09; Q9Y650; B4DJX0; B5MCC1; B9A0H6[Other Products]
UniProt Related Accession #
Q9HC10[Other Products]
Molecular Weight
226,535 Da
NCBI Official Full Name
otoferlin isoform e
NCBI Official Synonym Full Names
otoferlin
NCBI Official Symbol
OTOF??[Similar Products]
NCBI Official Synonym Symbols
AUNB1; DFNB6; DFNB9; NSRD9; FER1L2
??[Similar Products]
NCBI Protein Information
otoferlin
UniProt Protein Name
Otoferlin
UniProt Synonym Protein Names
Fer-1-like protein 2
UniProt Gene Name
OTOF??[Similar Products]
UniProt Synonym Gene Names
FER1L2??[Similar Products]
UniProt Entry Name
OTOF_HUMAN
NCBI Summary for OTOF
Mutations in this gene are a cause of neurosensory nonsyndromic recessive deafness, DFNB9. The short form of the encoded protein has 3 C2 domains, a single carboxy-terminal transmembrane domain found also in the C. elegans spermatogenesis factor FER-1 and human dysferlin, while the long form has 6 C2 domains. The homology suggests that this protein may be involved in vesicle membrane fusion. Several transcript variants encoding multiple isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Comments for OTOF
OTOF: Key calcium ion sensor involved in the Ca(2+)-triggered synaptic vesicle-plasma membrane fusion and in the control of neurotransmitter release at these output synapses. Interacts in a calcium-dependent manner to the presynaptic SNARE proteins at ribbon synapses of cochlear inner hair cells (IHCs) to trigger exocytosis of neurotransmitter. Also essential to synaptic exocytosis in immature outer hair cells (OHCs). May also play a role within the recycling of endosomes. Defects in OTOF are the cause of deafness autosomal recessive type 9 (DFNB9). DFNB9 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information. Defects in OTOF are a cause of auditory neuropathy, autosomal recessive, type 1 (AUNB1). A form of sensorineural hearing loss with absent or severely abnormal auditory brainstem response, in the presence of normal cochlear outer hair cell function and normal otoacoustic emissions. Auditory neuropathies result from a lesion in the area including the inner hair cells, connections between the inner hair cells and the cochlear branch of the auditory nerve, the auditory nerve itself and auditory pathways of the brainstem. In some cases AUNB1 phenotype can be temperature sensitive. Belongs to the ferlin family. 5 isoforms of the human protein are produced by alternative splicing.
Protein type: Vesicle; Membrane protein, integral
Chromosomal Location of Human Ortholog: 2p23.1
Cellular Component: endoplasmic reticulum membrane; synaptic vesicle membrane; basolateral plasma membrane; integral to membrane; cytosol; cell junction
Molecular Function: calcium ion binding
Biological Process: synaptic vesicle exocytosis; sensory perception of sound
Disease: Deafness, Autosomal Recessive 9
Research Articles on OTOF
1. Our observation of the discordant audiologic phenotype within the same DFNB9 family is more likely due to the loss of OAE over time rather than a genotype-phenotype correlation.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
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