Product Name
INSRR, siRNA
Full Product Name
INSRR siRNA (Rat)
Product Synonym Names
Insulin receptor-related protein; IRR; IR-related receptor
Product Gene Name
INSRR sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q64716
Specificity
INSRR siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat INSRR gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of INSRR sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
INSRR sirna
siRNA to inhibit INSRR expression using RNA interference
Applications Tested/Suitable for INSRR sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for INSRR. It may not necessarily be applicable to this product.
NCBI Accession #
NP_071548.2
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NCBI GenBank Nucleotide #
NM_022212.2
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UniProt Primary Accession #
Q64716
[Other Products]
UniProt Secondary Accession #
Q63146[Other Products]
UniProt Related Accession #
Q64716[Other Products]
Molecular Weight
144,846 Da
NCBI Official Full Name
insulin receptor-related protein
NCBI Official Synonym Full Names
insulin receptor-related receptor
NCBI Official Symbol
Insrr??[Similar Products]
NCBI Official Synonym Symbols
Sirr
??[Similar Products]
NCBI Protein Information
insulin receptor-related protein
UniProt Protein Name
Insulin receptor-related protein
UniProt Synonym Protein Names
IR-related receptor
Protein Family
Insulin receptor-related protein
UniProt Gene Name
Insrr??[Similar Products]
UniProt Synonym Gene Names
IRR??[Similar Products]
UniProt Entry Name
INSRR_RAT
NCBI Summary for INSRR
member of the insulin receptor family [RGD, Feb 2006]
UniProt Comments for INSRR
IRR: Receptor with tyrosine-protein kinase activity. Functions as a pH sensing receptor which is activated by increased extracellular pH. Activates an intracellular signaling pathway that involves IRS1 and AKT1/PKB. Probable tetramer of 2 alpha and 2 beta chains linked by disulfide bonds. The alpha chains contribute to the formation of the ligand-binding domain, while the beta chains carry the kinase domain. Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
Protein type: Protein kinase, TK; Protein kinase, tyrosine (receptor); Kinase, protein; EC 2.7.10.1; Membrane protein, integral; TK group; InsR family
Cellular Component: integral to plasma membrane; receptor complex
Molecular Function: insulin receptor substrate binding; phosphoinositide 3-kinase binding; transmembrane receptor protein tyrosine kinase activity; ATP binding
Biological Process: peptidyl-tyrosine phosphorylation; male sex determination; protein amino acid autophosphorylation; actin cytoskeleton reorganization; transmembrane receptor protein tyrosine kinase signaling pathway
Research Articles on INSRR
1. the influence of a stimulatable IRR-tyrosine kinase on major IR/IGF1R signaling pathways and on proliferation and apoptosis of INS-1E beta-cells
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
