Full Product Name
Goat anti-c-Myb (aa557-569) Antibody
Product Synonym Names
Goat Anti-c-MYB Antibody; MYB; v-myb myeloblastosis viral oncogene homolog (avian); Cmyb; c-myb; c-myb_CDS; efg; c-myb protein (140 AA); c-myb10A_CDS; c-myb13A_CDS; c-myb14A_CDS; c-myb8B_CDS; v-myb myeloblastosis viral oncogene homolog; Cmyb antibody; c-myb antibody; c-myb protein (140 AA) antibody; c-myb_CDS antibody; c-myb10A_CDS antibody; c-myb13A_CDS antibody; c-myb14A_CDS antibody; c-myb8B_CDS antibody; efg antibody; v-myb myeloblastosis viral oncogene homolog antibody; v-myb myeloblastosis viral oncogene homolog (avian) antibody; MYB antibody; c-Myb (aa557-569)
Product Gene Name
anti-MYB antibody
[Similar Products]
Product Synonym Gene Name
c-Myb[Similar Products]
Antibody/Peptide Pairs
c-Myb peptide (MBS427353) is used for blocking the activity of c-Myb antibody (MBS422371)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence
HRDQTVKTQK ENT
Species Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow, Pig
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (lot specific)
Immunogen
Peptide with sequence C-HRDQTVKTQKENT, from the internal region of the protein sequence according to NP_001123645.1; NP_005366.2; NP_001123644.1; NP_001155128.1; NP_001155129.1; NP_001155130.1; NP_001155131.1; NP_001155132.1.
Note
This antibody is expected to recognize all reported isofoms. (NP_001123645.1; NP_005366.2; NP_001123644.1; NP_001155128.1; NP_001155129.1; NP_001155130.1; NP_001155131.1; NP_001155132.1).
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of anti-MYB antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-MYB antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes for anti-MYB antibody
Peptide ELISA: Antibody detection limit dilution 1: 8000.
Western Blot: Approx 85kDa band observed in lysates of cell line Jurkat (calculated MW of 85.5kDa according to NP_001123645.1). Recommended concentration: 0.5-1.5ug/ml.
Western Blot (WB) of anti-MYB antibody
(0.5ug/ml) staining of Jurkat lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
NCBI/Uniprot data below describe general gene information for MYB. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001123645.1
[Other Products]
NCBI Related Accession #
Manufactured in an ISO 9001:2008 Certified Laboratory.NP_005366.2; NP_001123644.1; NP_001155128.1; NP_001155129.1; NP_001155130.1; NP_001155131.1; NP_001155132.1[Other Products]
NCBI GenBank Nucleotide #
NM_001130173.1
[Other Products]
UniProt Secondary Accession #
P78391; P78392; P78525; P78526; Q14023; Q14024; E9PI07; E9PLZ5; E9PNA4; E9PNL6; E9PRS2[Other Products]
UniProt Related Accession #
P10242[Other Products]
Molecular Weight
37,507 Da
NCBI Official Full Name
transcriptional activator Myb isoform 1
NCBI Official Synonym Full Names
MYB proto-oncogene, transcription factor
NCBI Official Symbol
MYB??[Similar Products]
NCBI Official Synonym Symbols
efg; Cmyb; c-myb; c-myb_CDS
??[Similar Products]
NCBI Protein Information
transcriptional activator Myb
UniProt Protein Name
Transcriptional activator Myb
UniProt Synonym Protein Names
Proto-oncogene c-Myb
Protein Family
Myb protein
UniProt Gene Name
MYB??[Similar Products]
UniProt Entry Name
MYB_HUMAN
NCBI Summary for MYB
This gene encodes a protein with three HTH DNA-binding domains that functions as a transcription regulator. This protein plays an essential role in the regulation of hematopoiesis. This gene may be aberrently expressed or rearranged or undergo translocation in leukemias and lymphomas, and is considered to be an oncogene. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016]
UniProt Comments for MYB
Myb: Transcriptional activator; DNA-binding protein that specifically recognize the sequence 5'-YAAC[GT]G-3'. Plays an important role in the control of proliferation and differentiation of hematopoietic progenitor cells. 6 isoforms of the human protein are produced by alternative splicing.
Protein type: Oncoprotein; Transcription factor; DNA-binding
Chromosomal Location of Human Ortholog: 6q22-q23
Cellular Component: nuclear matrix
Molecular Function: protein binding
Biological Process: chromatin remodeling; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; positive regulation of histone H3-K4 methylation; positive regulation of histone H3-K9 methylation; positive regulation of T-helper cell differentiation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; regulation of transcription, DNA-dependent; transcription from RNA polymerase II promoter
Research Articles on MYB
1. MYB is aberrantly overexpressed in PC cells and acts as a key determinant of pancreatic tumour growth and metastasis
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
