Full Product Name
ApoE Polyclonal Antibody
Product Synonym Names
AD2; APO-E; ApoE4; LDLCQ5; LPG
Product Gene Name
anti-ApoE antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence
GPLVEQGRVR AATVGSLAGQ PLQERAQAWG ERLRARMEEM GSRTRDRLDE VKEQVAEVRA KLEEQAQQIR LQAEAFQARL KSWFEPLVED MQRQWAGLVE K
3D Structure
ModBase 3D Structure for P02649
Species Reactivity
Human, Mouse, Rat
Purity/Purification
Affinity Purification
Immunogen
A synthetic peptide of human ApoE
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Cellular Location
Secreted
Positive Samples
LO2, A-549, SKOV3, 293T, Mouse brain, Mouse liver, Rat liver, Rat ovary
Preparation and Storage
Store at -20 degree C. Avoid freeze / thaw cycles.
Other Notes
Small volumes of anti-ApoE antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-ApoE antibody
The protein encoded by this gene is a major apoprotein of the chylomicron. It binds to a specific liver and peripheral cell receptor, and is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. This gene maps to chromosome 19 in a cluster with the related apolipoprotein C1 and C2 genes. Mutations in this gene result in familial dysbetalipoproteinemia, or type III hyperlipoproteinemia (HLP III), in which increased plasma cholesterol and triglycerides are the consequence of impaired clearance of chylomicron and VLDL remnants.
Product Categories/Family for anti-ApoE antibody
Polyclonal Antibodies
Applications Tested/Suitable for anti-ApoE antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-ApoE antibody
WB: 1:500 - 1:2000
IHC: 1:50 - 1:100
Western Blot (WB) of anti-ApoE antibody
Western blot analysis of extracts of various cell lines, using ApoE antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 3s.
Immunohistochemistry (IHC) of anti-ApoE antibody
Immunohistochemistry of paraffin-embedded human prostate using ApoE antibody at dilution of 1:100 (40x lens).
NCBI/Uniprot data below describe general gene information for ApoE. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000032.1
[Other Products]
NCBI GenBank Nucleotide #
NM_000041.3
[Other Products]
UniProt Primary Accession #
P02649
[Other Products]
UniProt Secondary Accession #
Q9P2S4; B2RC15; C0JYY5[Other Products]
UniProt Related Accession #
P02649[Other Products]
Molecular Weight
Calculated: 36kDa
Observed: 50kDa
NCBI Official Full Name
apolipoprotein E isoform b
NCBI Official Synonym Full Names
apolipoprotein E
NCBI Official Symbol
APOE??[Similar Products]
NCBI Official Synonym Symbols
AD2; LPG; APO-E; ApoE4; LDLCQ5
??[Similar Products]
NCBI Protein Information
apolipoprotein E
UniProt Protein Name
Apolipoprotein E
Protein Family
Apolipoprotein
UniProt Gene Name
APOE??[Similar Products]
UniProt Synonym Gene Names
Apo-E??[Similar Products]
NCBI Summary for ApoE
The protein encoded by this gene is a major apoprotein of the chylomicron. It binds to a specific liver and peripheral cell receptor, and is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. This gene maps to chromosome 19 in a cluster with the related apolipoprotein C1 and C2 genes. Mutations in this gene result in familial dysbetalipoproteinemia, or type III hyperlipoproteinemia (HLP III), in which increased plasma cholesterol and triglycerides are the consequence of impaired clearance of chylomicron and VLDL remnants. [provided by RefSeq, Jun 2016]
UniProt Comments for ApoE
Mediates the binding, internalization, and catabolism of lipoprotein particles. It can serve as a ligand for the LDL (apo B/E) receptor and for the specific apo-E receptor (chylomicron remnant) of hepatic tissues.
Research Articles on ApoE
1. Study elucidated that the APOE4 epsilon4 allele can enhance AD susceptibility and promotes the expressions of inflammatory factors in Alzheimer's disease (AD). Additionally, the epsilon3/4 genotype and TNF-alpha, IL-6, and IL-1beta levels were associated with AD susceptibility in the Han population, while the epsilon3/4 and epsilon4/4 genotypes and TNF-alpha, IL-6, and IL-1beta levels were associated with AD susceptibi...
Precautions
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Disclaimer
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