Product Name
PABPN1, siRNA
Full Product Name
PABPN1 siRNA (Human)
Product Synonym Names
PAB2; PABP2; Polyadenylate-binding protein 2; PABP-2; Poly(A)-binding protein 2; Nuclear poly(A)-binding protein 1; Poly(A)-binding protein II; PABII; Polyadenylate-binding nuclear protein 1
Product Gene Name
PABPN1 sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q86U42
Specificity
PABPN1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human PABPN1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of PABPN1 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
PABPN1 sirna
siRNA to inhibit PABPN1 expression using RNA interference
Applications Tested/Suitable for PABPN1 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for PABPN1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_004634.1
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NCBI GenBank Nucleotide #
NM_004643.3
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UniProt Primary Accession #
Q86U42
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UniProt Secondary Accession #
O43484; D3DS49[Other Products]
UniProt Related Accession #
Q86U42[Other Products]
Molecular Weight
37,171 Da
NCBI Official Full Name
polyadenylate-binding protein 2
NCBI Official Synonym Full Names
poly(A) binding protein, nuclear 1
NCBI Official Symbol
PABPN1??[Similar Products]
NCBI Official Synonym Symbols
OPMD; PAB2; PABII; PABP2; PABP-2
??[Similar Products]
NCBI Protein Information
polyadenylate-binding protein 2
UniProt Protein Name
Polyadenylate-binding protein 2
UniProt Synonym Protein Names
Nuclear poly(A)-binding protein 1; Poly(A)-binding protein II; PABII; Polyadenylate-binding nuclear protein 1
UniProt Gene Name
PABPN1??[Similar Products]
UniProt Synonym Gene Names
PAB2; PABP2; PABP-2; Poly(A)-binding protein 2; PABII??[Similar Products]
UniProt Entry Name
PABP2_HUMAN
NCBI Summary for PABPN1
This gene encodes an abundant nuclear protein that binds with high affinity to nascent poly(A) tails. The protein is required for progressive and efficient polymerization of poly(A) tails at the 3' ends of eukaryotic transcripts and controls the size of the poly(A) tail to about 250 nt. At steady-state, this protein is localized in the nucleus whereas a different poly(A) binding protein is localized in the cytoplasm. This gene contains a GCG trinucleotide repeat at the 5' end of the coding region, and expansion of this repeat from the normal 6 copies to 8-13 copies leads to autosomal dominant oculopharyngeal muscular dystrophy (OPMD) disease. Related pseudogenes have been identified on chromosomes 19 and X. Read-through transcription also exists between this gene and the neighboring upstream BCL2-like 2 (BCL2L2) gene. [provided by RefSeq, Dec 2010]
UniProt Comments for PABPN1
PABPN1: Involved in the 3'-end formation of mRNA precursors (pre-mRNA) by the addition of a poly(A) tail of 200-250 nt to the upstream cleavage product. Stimulates poly(A) polymerase (PAPOLA) conferring processivity on the poly(A) tail elongation reaction and controls also the poly(A) tail length. Increases the affinity of poly(A) polymerase for RNA. Is also present at various stages of mRNA metabolism including nucleocytoplasmic trafficking and nonsense-mediated decay (NMD) of mRNA. Cooperates with SKIP to synergistically activate E-box-mediated transcription through MYOD1 and may regulate the expression of muscle-specific genes. Binds to poly(A) and to poly(G) with high affinity. May protect the poly(A) tail from degradation. Defects in PABPN1 are the cause of oculopharyngeal muscular dystrophy (OPMD). OPMD is a form of late- onset slowly progressive myopathy characterized by eyelid ptosis, dysphagia and, sometimes by other cranial and limb-muscle involvement. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: RNA-binding; RNA splicing
Chromosomal Location of Human Ortholog: 14q11.2
Cellular Component: nucleoplasm; cytoplasm; nucleus; ribonucleoprotein complex
Molecular Function: protein binding; RNA binding; nucleotide binding
Biological Process: transcription from RNA polymerase II promoter; RNA splicing; modification by virus of host mRNA processing; viral infectious cycle; nuclear mRNA splicing, via spliceosome; RNA processing; poly(A)+ mRNA export from nucleus; modification by virus of host cellular process; muscle contraction; virus-host interaction; gene expression; mRNA 3'-end processing; termination of RNA polymerase II transcription
Disease: Oculopharyngeal Muscular Dystrophy
Research Articles on PABPN1
1. The ability of PABPN1 to promote splicing requires its RNA binding and, to a lesser extent, poly(A)polymerase (PAP) - stimulatory functions.
Precautions
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Disclaimer
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