Product Name
ACADS, siRNA
Full Product Name
ACADS siRNA (Rat)
Product Synonym Names
Short-chain specific acyl-CoA dehydrogenase mitochondrial; SCAD; Butyryl-CoA dehydrogenase
Product Gene Name
ACADS sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P15651
Specificity
ACADS siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat ACADS gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of ACADS sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
ACADS sirna
siRNA to inhibit ACADS expression using RNA interference
Applications Tested/Suitable for ACADS sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for ACADS. It may not necessarily be applicable to this product.
NCBI Accession #
NP_071957.1
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NCBI GenBank Nucleotide #
NM_022512.2
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UniProt Primary Accession #
P15651
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UniProt Related Accession #
P15651[Other Products]
Molecular Weight
44,765 Da
NCBI Official Full Name
short-chain specific acyl-CoA dehydrogenase, mitochondrial
NCBI Official Synonym Full Names
acyl-CoA dehydrogenase, C-2 to C-3 short chain
NCBI Official Symbol
Acads??[Similar Products]
NCBI Official Synonym Symbols
Scad
??[Similar Products]
NCBI Protein Information
short-chain specific acyl-CoA dehydrogenase, mitochondrial
UniProt Protein Name
Short-chain specific acyl-CoA dehydrogenase, mitochondrial
UniProt Synonym Protein Names
Butyryl-CoA dehydrogenase
Protein Family
Short-chain specific acyl-CoA dehydrogenase
UniProt Gene Name
Acads??[Similar Products]
UniProt Synonym Gene Names
SCAD??[Similar Products]
UniProt Entry Name
ACADS_RAT
UniProt Comments for ACADS
ACADS: Defects in ACADS are the cause of acyl-CoA dehydrogenase short-chain deficiency (ACADSD). It is an autosomal recessive disorder resulting in acute acidosis and muscle weakness in infants, and a form of lipid-storage myopathy in *****s. Belongs to the acyl-CoA dehydrogenase family.
Protein type: Lipid-binding; Mitochondrial; Oxidoreductase; Carbohydrate Metabolism - butanoate; Lipid Metabolism - fatty acid; EC 1.3.8.1; Amino Acid Metabolism - valine, leucine and isoleucine degradation
Cellular Component: mitochondrion; mitochondrial matrix; mitochondrial membrane; nucleus
Molecular Function: acyl-CoA dehydrogenase activity; butyryl-CoA dehydrogenase activity; FAD binding; acyl-CoA binding
Biological Process: response to starvation; fatty acid beta-oxidation; response to glucocorticoid stimulus; fatty acid beta-oxidation using acyl-CoA dehydrogenase; protein homotetramerization; butyrate catabolic process
Research Articles on ACADS
1. the phosphorylation of ERK1/2 inhibited the expression and activity of SCAD through the PPARalpha signaling pathway, which induced the development of pathological cardiomyocyte hypertrophy.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
