Anti -Sarcoglycan, delta

For Research Use Only. Not for use in diagnostic procedures.

Chromosome: 5; NC_000005.9 (155753767..156194799). Location: 5q33-q34

Polyclonal

IgG

Goat

Recognizes human delta-Sarcoglycan extracellular domain.

Affinity Purified
Purified by immunoaffinity chromatography.

Supplied as a lyophilized powder from PBS, pH 7.2, 5% trehalose. Reconstitute with 500ul 40-50% glycerol, PBS.

Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute by adding sterile 40-50% glycerol, PBS. Aliquot and store at -20 degree C. Reconstituted product is stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Small volumes of anti-SGCD antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Delta-sarcoglycan is one of six known sarcoglycans, which are type I (alpha and epsilon) or type II (beta, gamma, delta or zeta) transmembrane glycoproteins. Sarcoglycans associate in different combinations in a tissue-specific manner, to form heterotetrameric, pentameric and hexameric membrane glycoprotein complexes (SGC). SGC interacts laterally with the dystroglycan complex, and both complexes are components of the dystrophin-associated glycoprotein complex (DGC), which forms a link between the cytoskeleton and the extracellular matrix. Mutations in sarcoglycans are associated with recessive autosomally inherited muscular dystrophy.

Antibodies; Abs to Extracellular Matrix Proteins

ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)

Suitable for use in Direct ELISA, Western Blot and Immunohistochemistry.
Dilution: Direct ELISA: 0.5-1.0ug/ml; Detection limit is ~0.3ng/well
Western Blot: 0.1-0.2ug/ml; Detection limit is ~5ng/lane (non-reducing) and 1ng/lane (reducing)
Immunohistochemistry: 10ug/ml; Detects delta-Sarcoglycan in human fetal heart tissue sections. Sections were fixed with PBS, 10% normal donkey serum, 0.1% Triton X-100, and 1% BSA for 45 minutes at RT. After blocking, cells were incubated with diluted S0160 overnight at 4 degree C followed by Rhodamine Red-coupled anti-goat IgG at RT in the dark for 1 hr. Between each step, cells were washed with PBS, 1% BSA.

32,071 Da[Similar Products]

sarcoglycan, delta (35kDa dystrophin-associated glycoprotein)

delta-sarcoglycan; delta-SG; OTTHUMP00000223946; OTTHUMP00000223947; OTTHUMP00000223961; placental delta sarcoglycan; 35kD dystrophin-associated glycoprotein; 35 kDa dystrophin-associated glycoprotein; dystrophin associated glycoprotein, delta sarcoglycan

Delta-sarcoglycan

SGCD_HUMAN

The protein encoded by this gene is one of the four known components of the sarcoglycan complex, which is a subcomplex of the dystrophin-glycoprotein complex (DGC). DGC forms a link between the F-actin cytoskeleton and the extracellular matrix. This protein is expressed most abundantly in skeletal and cardiac muscle. Mutations in this gene have been associated with autosomal recessive limb-girdle muscular dystrophy and dilated cardiomyopathy. Alternatively spliced transcript variants encoding distinct isoforms have been observed for this gene. [provided by RefSeq]

SGCD: Component of the sarcoglycan complex, a subcomplex of the dystrophin-glycoprotein complex which forms a link between the F-actin cytoskeleton and the extracellular matrix. Defects in SGCD are the cause of limb-girdle muscular dystrophy type 2F (LGMD2F). LGMD2F is an autosomal recessive disorder. Defects in SGCD are the cause of cardiomyopathy dilated type 1L (CMD1L). Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death. Belongs to the sarcoglycan beta/delta/gamma/zeta family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Dystrophin complex; Membrane protein, integral

Chromosomal Location of Human Ortholog: 5q33-q34

Cellular Component: dystrophin-associated glycoprotein complex; cytoskeleton; cytoplasm; plasma membrane; integral to membrane; sarcoglycan complex; sarcolemma

Biological Process: cardiac muscle development; muscle development; heart contraction; muscle cell development

Disease: Muscular Dystrophy, Limb-girdle, Type 2f; Cardiomyopathy, Dilated, 1l

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