Product Name
Non-homologous end-joining factor 1 (Nhej1), Recombinant Protein
Full Product Name
Recombinant Mouse Non-homologous end-joining factor 1 (Nhej1)
Product Gene Name
Nhej1 recombinant protein
[Similar Products]
Product Synonym Gene Name
Nhej1[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence Positions
1-295aa, full length protein
Sequence
MEELEQDLLL QPWAWLQLAE NSLLAKVSIT KHGYALLISD LQQVWHEQVD TSVVSQRAKE LNKRLTAPPA ALLCHLDEAL RPLFKDSAHP SKATFSCDRG EEGLILRVQS ELSGLPFSWH FHCIPASSSL VSQHLIHPLM GVSLALQSHV RELAALLRMK DLEIQAYQES GAVLSRSRLK TEPFEENSFL EQFMAEKLPE ACAVGDGKPF AMSLQSLYVA VTKQQIQARQ AHKDSGETQA SSSTSPRGTD NQPEEPVSLS STLSEPEYEP VAASGPMHRA RLVKSKRKKP RGLFS
3D Structure
ModBase 3D Structure for Q3KNJ2
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Purity/Purification
>85% (SDS-PAGE) (lot specific)
Form/Format
Liquid containing glycerol
Tag Information
This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.
Sterility
Sterile filter available upon request.
Endotoxin
Low endotoxin available upon request.
Storage Buffer
Tris-based buffer, 50% glycerol
Preparation and Storage
Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Other Notes
Small volumes of Nhej1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
Nhej1 recombinant protein
Double-strand breaks in DNA result from genotoxic stresses and are among the most damaging of DNA lesions. This gene encodes a DNA repair factor essential for the nonhomologous end-joining pathway, which preferentially mediates repair of double-stranded breaks. Mutations in this gene cause different kinds of severe combined immunodeficiency disorders.
NCBI/Uniprot data below describe general gene information for Nhej1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_083618.3
[Other Products]
NCBI GenBank Nucleotide #
NM_029342.4
[Other Products]
UniProt Primary Accession #
Q3KNJ2
[Other Products]
UniProt Secondary Accession #
Q99JK0; Q9D9U0; A2RT39[Other Products]
UniProt Related Accession #
Q3KNJ2[Other Products]
Molecular Weight
25,233 Da
NCBI Official Full Name
non-homologous end-joining factor 1
NCBI Official Synonym Full Names
nonhomologous end-joining factor 1
NCBI Official Symbol
Nhej1??[Similar Products]
NCBI Official Synonym Symbols
XLF; cernunnos; 1700029B21Rik
??[Similar Products]
NCBI Protein Information
non-homologous end-joining factor 1
UniProt Protein Name
Non-homologous end-joining factor 1
UniProt Synonym Protein Names
Protein cernunnos; XRCC4-like factor
Protein Family
Non-homologous end-joining factor
UniProt Gene Name
Nhej1??[Similar Products]
UniProt Synonym Gene Names
Xlf??[Similar Products]
UniProt Comments for Nhej1
DNA repair protein involved in DNA nonhomologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. May serve as a bridge between XRCC4 and the other NHEJ factors located at DNA ends, or may participate in reconfiguration of the end bound NHEJ factors to allow XRCC4 access to the DNA termini. It may act in concert with XRCC6/XRCC5 (Ku) to stimulate XRCC4-mediated joining of blunt ends and several types of mismatched ends that are noncomplementary or partially complementary (PubMed:17360556). Binds DNA in a length-dependent manner ().
Research Articles on Nhej1
1. we also find that PAXX deficiency has no impact on V(D)J recombination DSB joining in ATM-deficient pro-B lines. We discuss implications of these findings with respect to potential PAXX and XLF functions in C-NHEJ.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
