Product Name
DHCR24, siRNA
Full Product Name
DHCR24 siRNA (Human)
Product Synonym Names
KIAA0018; Delta(24)-sterol reductase; 24-dehydrocholesterol reductase; 3-beta-hydroxysterol delta-24-reductase; Diminuto/dwarf1 homolog; Seladin-1
Product Gene Name
DHCR24 sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q15392
Specificity
DHCR24 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human DHCR24 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of DHCR24 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
DHCR24 sirna
siRNA to inhibit DHCR24 expression using RNA interference
Applications Tested/Suitable for DHCR24 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for DHCR24. It may not necessarily be applicable to this product.
NCBI Accession #
NP_055577.1
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NCBI GenBank Nucleotide #
NM_014762.3
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UniProt Primary Accession #
Q15392
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UniProt Secondary Accession #
Q9HBA8; B7Z817; D3DQ51[Other Products]
UniProt Related Accession #
Q15392[Other Products]
Molecular Weight
54,750 Da
NCBI Official Full Name
delta(24)-sterol reductase
NCBI Official Synonym Full Names
24-dehydrocholesterol reductase
NCBI Official Symbol
DHCR24??[Similar Products]
NCBI Official Synonym Symbols
DCE; SELADIN1; Nbla03646; seladin-1
??[Similar Products]
NCBI Protein Information
delta(24)-sterol reductase
UniProt Protein Name
Delta(24)-sterol reductase
UniProt Synonym Protein Names
24-dehydrocholesterol reductase; 3-beta-hydroxysterol delta-24-reductase; Diminuto/dwarf1 homolog; Seladin-1
Protein Family
Delta(24)-sterol reductase
UniProt Gene Name
DHCR24??[Similar Products]
UniProt Synonym Gene Names
KIAA0018??[Similar Products]
UniProt Entry Name
DHC24_HUMAN
NCBI Summary for DHCR24
This gene encodes a flavin adenine dinucleotide (FAD)-dependent oxidoreductase which catalyzes the reduction of the delta-24 double bond of sterol intermediates during cholesterol biosynthesis. The protein contains a leader sequence that directs it to the endoplasmic reticulum membrane. Missense mutations in this gene have been associated with desmosterolosis. Also, reduced expression of the gene occurs in the temporal cortex of Alzheimer disease patients and overexpression has been observed in adrenal gland cancer cells. [provided by RefSeq, Jul 2008]
UniProt Comments for DHCR24
Diminuto: the enzyme 3-beta-hydroxysterol delta-24-reductase (DHCR24), a member of the flavin adenine dinucleotide (FAD)-dependent oxidoreductases, catalyzes the reduction of the delta-24 double bond of sterol intermediates during cholesterol biosynthesis. Highly expressed in brain and adrenal gland with moderate expression in liver, lung, spleen, prostate and spinal cord. Low expression in heart, uterus and prostate. Undetectable in blood cells. Overexpressed in the adenoma tissue of patients with Cushing's syndrome. May be involved in the molecular events of adrenocortical tumorigenesis by facilitating steroid synthesis and cell growth.
Protein type: EC 1.3.1.72; Lipid Metabolism - steroid biosynthesis; Membrane protein, integral; Oxidoreductase
Chromosomal Location of Human Ortholog: 1p32.3
Cellular Component: Golgi membrane; endoplasmic reticulum membrane; cytoskeleton; membrane; endoplasmic reticulum; integral to membrane; nucleus; cytosol
Molecular Function: delta24(24-1) sterol reductase activity; UDP-N-acetylmuramate dehydrogenase activity; enzyme binding; peptide antigen binding; FAD binding; oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor; delta24-sterol reductase activity
Biological Process: skin development; tissue development; apoptosis; response to hormone stimulus; negative regulation of caspase activity; cholesterol biosynthetic process; negative regulation of cell proliferation; amyloid precursor protein catabolic process; protein localization; Ras protein signal transduction; male genitalia development; response to oxidative stress; cell cycle arrest; plasminogen activation; negative regulation of apoptosis
Disease: Desmosterolosis
Research Articles on DHCR24
1. Data (including data from studies using RNA from post-mortem brain tissue) suggest that expression of DHCR24 is up-regulated in parietal cortex of patients with Huntington's disease as compared to control subjects.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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