Product Name
DHCR24, Polyclonal Antibody
Full Product Name
DHCR24 Antibody
Product Synonym Names
DCE; SELADIN1; Nbla03646; seladin-1
Product Gene Name
anti-DHCR24 antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q15392
Species Reactivity
Human, Mouse
Specificity
The antibody detects endogenous levels of total DHCR24 protein.
Purity/Purification
Antigen Affinity Purification
Form/Format
Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
Concentration
2.1 mg/ml (lot specific)
Immunogen
Fusion protein of human DHCR24
Preparation and Storage
Store at -20 degree C
Other Notes
Small volumes of anti-DHCR24 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-DHCR24 antibody
This gene encodes a flavin adenine dinucleotide (FAD)-dependent oxidoreductase which catalyzes the reduction of the delta-24 double bond of sterol intermediates during cholesterol biosynthesis. The protein contains a leader sequence that directs it to the endoplasmic reticulum membrane. Missense mutations in this gene have been associated with desmosterolosis.
Product Categories/Family for anti-DHCR24 antibody
Total protein Ab
Applications Tested/Suitable for anti-DHCR24 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-DHCR24 antibody
Western Blot: 1:500-1:2000
Immunohistochemistry: 1:25-1:100
Western Blot (WB) of anti-DHCR24 antibody
Gel: 8%SDS-PAGE Lysate: 80 μ g Lane: Mouse brain tissue Primary antibody: 1/400 dilution Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 5 seconds
Immunohistochemistry (IHC) of anti-DHCR24 antibody
Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue using at dilution 1/35.
Immunohistochemistry (IHC) of anti-DHCR24 antibody
Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue using at dilution 1/35.
NCBI/Uniprot data below describe general gene information for DHCR24. It may not necessarily be applicable to this product.
NCBI Accession #
NP_055577.1
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NCBI GenBank Nucleotide #
NM_014762.3
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UniProt Primary Accession #
Q15392
[Other Products]
UniProt Secondary Accession #
Q9HBA8; B7Z817; D3DQ51[Other Products]
UniProt Related Accession #
Q15392[Other Products]
Molecular Weight
54,750 Da
NCBI Official Full Name
delta(24)-sterol reductase
NCBI Official Synonym Full Names
24-dehydrocholesterol reductase
NCBI Official Symbol
DHCR24??[Similar Products]
NCBI Official Synonym Symbols
DCE; SELADIN1; Nbla03646; seladin-1
??[Similar Products]
NCBI Protein Information
delta(24)-sterol reductase
UniProt Protein Name
Delta(24)-sterol reductase
UniProt Synonym Protein Names
24-dehydrocholesterol reductase; 3-beta-hydroxysterol delta-24-reductase; Diminuto/dwarf1 homolog; Seladin-1
Protein Family
Delta(24)-sterol reductase
UniProt Gene Name
DHCR24??[Similar Products]
UniProt Synonym Gene Names
KIAA0018??[Similar Products]
UniProt Entry Name
DHC24_HUMAN
UniProt Comments for DHCR24
Diminuto: the enzyme 3-beta-hydroxysterol delta-24-reductase (DHCR24), a member of the flavin adenine dinucleotide (FAD)-dependent oxidoreductases, catalyzes the reduction of the delta-24 double bond of sterol intermediates during cholesterol biosynthesis. Highly expressed in brain and adrenal gland with moderate expression in liver, lung, spleen, prostate and spinal cord. Low expression in heart, uterus and prostate. Undetectable in blood cells. Overexpressed in the adenoma tissue of patients with Cushing's syndrome. May be involved in the molecular events of adrenocortical tumorigenesis by facilitating steroid synthesis and cell growth.
Protein type: EC 1.3.1.72; Lipid Metabolism - steroid biosynthesis; Membrane protein, integral; Oxidoreductase
Chromosomal Location of Human Ortholog: 1p32.3
Cellular Component: endoplasmic reticulum; endoplasmic reticulum membrane; membrane; nucleus
Molecular Function: delta24(24-1) sterol reductase activity; delta24-sterol reductase activity; enzyme binding; oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor; peptide antigen binding
Biological Process: cholesterol biosynthetic process; cholesterol biosynthetic process via desmosterol; cholesterol biosynthetic process via lathosterol; negative regulation of apoptosis; negative regulation of caspase activity; response to oxidative stress; skin development; tissue development
Disease: Desmosterolosis
Precautions
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Disclaimer
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