TRH

For Research Use Only. Not for use in diagnostic procedures.

Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

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Small volumes of TRH elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS760732 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Thyrotropin Releasing Hormone (TRH) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing TRH. The ELISA analytical biochemical technique of the MBS760732 kit is based on TRH antibody-TRH antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TRH antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TRH. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Principle of the Assay||This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with Human TRH. During the reaction,Human TRH in the sample or standard competes with a fixed amount of Human TRH on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to Human TRH. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Human TRH in the samples is then determined by comparing the O.D. of the samples to the standard curve.

27,404 Da

thyrotropin releasing hormone

thyrotropin releasing hormone

Pro-thyrotropin-releasing hormone

Pro-TRH; TRH; TRF??[Similar Products]

TRH_HUMAN

This gene encodes a member of the thyrotropin-releasing hormone family. Cleavage of the encoded proprotein releases mature thyrotropin-releasing hormone, which is a tripeptide hypothalamic regulatory hormone. The human proprotein contains six thyrotropin-releasing hormone tripeptides. Thyrotropin-releasing hormone is involved in the regulation and release of thyroid-stimulating hormone, as well as prolactin. Deficiency of this hormone has been associated with hypothalamic hypothyroidism. [provided by RefSeq, May 2013]

TRH: Functions as a regulator of the biosynthesis of TSH in the anterior pituitary gland and as a neurotransmitter/ neuromodulator in the central and peripheral nervous systems. May promote hair shaft elongation, prolonge the hair cycle growth phase (anagen) and antagonized its termination by TGFB2. May also increase proliferation and inhibited apoptosis of hair matrix keratinocytes. Belongs to the TRH family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 3q13.3-q21

Cellular Component: extracellular region; nucleus; plasma membrane; secretory granule

Molecular Function: neuropeptide hormone activity; thyrotropin-releasing hormone activity

Biological Process: ***** walking behavior; cell-cell signaling; eating behavior; histamine metabolic process; hormone-mediated signaling; negative regulation of glutamate secretion; positive regulation of gamma-aminobutyric acid secretion; positive regulation of insulin secretion; response to cold; response to corticosterone stimulus; response to ethanol; response to glucose stimulus; response to hypoxia; signal transduction

Disease: Thyrotropin-releasing Hormone Deficiency

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